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|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||An 18 amino acid peptide near the carboxy terminus of human SPRYD2.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||20 ug/ml|
|Immunofluorescence (IF)||20 µg/ml|
|Immunohistochemistry (IHC)||2.5 µg/ml|
|Western Blot (WB)||1-2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control is mouse heart tissue lysate.
PA5-21170 can be used with blocking peptide PEP-1284.
SPRYD2, also known as Myospryn, was originally identified as the muscle-specific partner of dysbindin and as a Mef-2 target gene. It is a large scaffolding protein localized to the Z-disc/costamere region of striated muscle. SPRYD2 includes a noncanonical tripartite motif (TRIM-like) that lacks the RING domain but consists of a B-box coiled coil (BBC), fibronectin 3 (FN3) repeats, and SPRY domains. SPRYD2 interacts with desmin and calcineurin, and it has been suggested to play a role in the biogenesis of lysosome and negatively regulates slow-fiber-type transformation and skeletal muscle regeneration. SPRYD2 is dysregulated in Duchenne muscular dystrophy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
2310076E16Rik; C5orf10, SPRYD2, TRIM76; cardiomyopathy associated 5; cardiomyopathy-associated protein 5; dystrobrevin-binding protein 2; genethonin-3; heart and skeletal muscle-specific and sprouty domain-containing; myospryn; SPRY domain-containing protein 2; stretch response protein 553; stretch-response protein 553; stretch-responsive fibronectin protein type 3; TRIM-like protein; tripartite motif-containing 76; tripartite motif-containing protein 76
2310076E16Rik; 2310076E21Rik; AA420382; AI504003; C5orf10; CMYA5; DTNBP2; SPRYD2; sr553; Srfsd; Tims; TRIM76