Proteintech

SSH3 Polyclonal Antibody

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SSH3 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of A431 cells using 18324-1-AP (SSH3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

SSH3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human placenta using 18324-1-AP (SSH3 antibody) at dilution of 1:100 (under 40x lens).

SSH3 Antibody in Western Blot (WB) — Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 18324-1-AP (SSH3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

SSH3 Antibody in Immunoprecipitation (IP) — IP result of anti-SSH3 (IP:18324-1-AP, 4ug; Detection:18324-1-AP 1:1000) with A431 cells lysate 2800ug.

Product Details

18324-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:4,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:10-1:100	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SSH3 Fusion Protein Ag13174 (310-659 aa encoded by BC004210) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.45 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: YRDFIDNQM LLLVAQRDRA SRIFPHLYLG SEWNAANLEE LQRNRVTHIL NMAREIDNFY PERFTYHNVR LWDEESAQLL PHWKETHRFI EAARAQGTHV LVHCKMGVSR SAATVLAYAM KQYECSLEQA LRHVQELRPI ARPNPGFLRQ LQIYQGILTA SRQSHVWEQK VGGVSPEEHP APEVSTPFPP LPPEPEGGGE EKVVGMEESQ AAPKEEPGPR PRINLRGVMR SISLLEPSLE LESTSETSDM PEVFSSHESS HEEPLQPFPQ LARTKGGQQV DRGPQPALKS RQSVVTLQGS AVVANRTQAF QEQEQGQGQG QGEPCISSTP RFRKVVRQAS VHDSGEEGEA (310-659 aa encoded by BC004210)

Target Information

The ADF (actin-depolymerizing factor)/cofilin family is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: hSSH-3L; Protein phosphatase Slingshot homolog 3; slingshot 3; slingshot homolog 3; slingshot-3L; slingshot-like 3; SSH-3L; SSH-like protein 3

Gene Aliases: BC028922; mSSH-3L; SSH-3; SSH3; SSH3L

UniProt ID: (Human) Q8TE77, (Mouse) Q8K330, (Rat) Q5XIS1

Entrez Gene ID: (Human) 54961, (Mouse) 245857, (Rat) 365396

Molecular Function: protein phosphatase