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Syntaxin 1A / Syntaxin 1B Monoclonal Antibody (2D3B5)

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Syntaxin 1A / Syntaxin 1B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human brain tissue slide using 66437-1-IG ( Syntaxin 1 Antibody) at dilution of 1:500 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Syntaxin 1A / Syntaxin 1B Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Syntaxin 1A / Syntaxin 1B antibody (Product # 66437-1-IG, Clone: 2D3B5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Syntaxin 1A / Syntaxin 1B Antibody in Western Blot (WB) — Fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66437-1-Ig (Syntaxin 1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Product Details

66437-1-IG

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:6,000	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunohistochemistry (PFA fixed) (IHC (PFA))	1:4,000	-
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Mouse, Rat
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	2D3B5
Immunogen	Syntaxin 1A / Syntaxin 1B Fusion Protein Ag10257 (1-263 aa encoded by BC062298) View immunogen
Conjugate	Unconjugated CoraLite488 CoraLite594
Form	Liquid
Concentration	1 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MKDRTQELR SAKDSDDEEE VVHVDRDHFM DEFFEQVEEI RGCIEKLSED VEQVKKQHSA ILAAPNPDEK TKQELEDLTA DIKKTANKVR SKLKAIEQSI EQEEGLNRSS ADLRIRKTQH STLSRKFVEV MTEYNATQSK YRDRCKDRIQ RQLEITGRTT TNEELEDMLE SGKLAIFTDD IKMDSQMTKQ ALNEIETRHN EIIKLETSIR ELHDMFVDMA MLVESQGEMI DRIEYNVEHS VDYVERAVSD TKKAVKYQSK ARRK (1-263 aa encoded by BC062298)

Target Information

This gene encodes a member of the syntaxin superfamily. Syntaxins are nervous system-specific proteins implicated in the docking of synaptic vesicles with the presynaptic plasma membrane. Syntaxins possess a single C-terminal transmembrane domain, a SNARE domain, and an N-terminal regulatory domain. Syntaxins bind synaptotagmin in a calcium-dependent fashion and interact with voltage dependent calcium and potassium channels via the C-terminal H3 domain. This gene product is a key molecule in ion channel regulation and synaptic exocytosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Munc18 and Munc13 serve as a functional template to orchestrate neuronal SNARE complex assembly.

Nature communications

Wang S,Li Y,Gong J,Ye S,Yang X,Zhang R,Ma C

Tue Jan 08 00:00:00 EST 2019

Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.

Nature communications

Lu IL,Chen C,Tung CY,Chen HH,Pan JP,Chang CH,Cheng JS,Chen YA,Wang CH,Huang CW,Kang YN,Chang HY,Li LL,Chang KP,Shih YH,Lin CH,Kwan SY,Tsai JW

Wed Jun 27 00:00:00 EDT 2018

Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.

Nature communications

Lu IL,Chen C,Tung CY,Chen HH,Pan JP,Chang CH,Cheng JS,Chen YA,Wang CH,Huang CW,Kang YN,Chang HY,Li LL,Chang KP,Shih YH,Lin CH,Kwan SY,Tsai JW

Wed Jun 27 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: adipocyte; adiponectin; FP17469; HGNC:11433; Neuron-specific antigen HPC-1; P35A; P35B; Synaptotagmin-associated 35 kDa protein; syntaxin 1 a; syntaxin 1A (brain); syntaxin 1A (brain)-like; syntaxin 1B1; syntaxin 1B2; Syntaxin 2; syntaxin a/b; Syntaxin-1A; Syntaxin-1B; Syntaxin-1B1; Syntaxin-1B2

Gene Aliases: GEFSP9; HPC-1; P35-1; Sap; STX1; STX1A; STX1B; STX1B1; STX1B2; Stx1bl; Stx2; SYN1A; Syn1b

UniProt ID: (Human) Q16623, (Human) P61266, (Mouse) O35526, (Rat) P32851, (Mouse) P61264, (Rat) P61265

Entrez Gene ID: (Human) 6804, (Human) 112755, (Mouse) 20907, (Rat) 116470, (Mouse) 56216, (Rat) 24923

Molecular Function: SNARE protein