Proteintech

THAP1 Polyclonal Antibody

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THAP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer using 12584-1-AP (THAP1 antibody) at dilution of 1:50 (under 40x lens).

THAP1 Antibody in Western Blot (WB) — Human brain tissue were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Product Details

12584-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 8 publications 8 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	THAP1 Fusion Protein Ag3285 (1-213 aa encoded by BC021721) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C

Product Specific Information

12584-1-AP is a rabbit polyclonal antibody raised against full-length of human THAP1.

Immunogen sequence: MVQSCSAYG CKNRYDKDKP VSFHKFPLTR PSLCKEWEAA VRRKNFKPTK YSSICSEHFT PDCFKRECNN KLLKENAVPT IFLCTEPHDK KEDLLEPQEQ LPPPPLPPPV SQVDAAIGLL MPPLQTPVNL SVFCDHNYTV EDTMHQRKRI HQLEQQVEKL RKKLKTAQQR CRRQERQLEK LKEVVHFQKE KDDVSERGYV ILPNDYFEIV EVPA (1-213 aa encoded by BC021721)

Target Information

DNA-binding transcription regulator that regulates endothelial cell proliferation and G1/S cell-cycle progression. Specifically binds the 5'-[AT]NTNN[GT]GGCA[AGT]-3' core DNA sequence and acts by modulating expression of pRB-E2F cell-cycle target genes, including RRM1. Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. May also have pro-apoptopic activity by potentiating both serum-withdrawal and TNF-induced apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The DYT6 Dystonia Protein THAP1 Regulates Myelination within the Oligodendrocyte Lineage.

Developmental cell

Yellajoshyula D,Liang CC,Pappas SS,Penati S,Yang A,Mecano R,Kumaran R,Jou S,Cookson MR,Dauer WT

Mon Jul 10 00:00:00 EDT 2017

Mutations in the histone methyltransferase gene KMT2B cause complex early-onset dystonia.

Nature genetics

Meyer E,Carss KJ,Rankin J,Nichols JM,Grozeva D,Joseph AP,Mencacci NE,Papandreou A,Ng J,Barral S,Ngoh A,Ben-Pazi H,Willemsen MA,Arkadir D,Barnicoat A,Bergman H,Bhate S,Boys A,Darin N,Foulds N,Gutowski N,Hills A,Houlden H,Hurst JA,Israel Z,Kaminska M,Limousin P,Lumsden D,McKee S,Misra S,Mohammed SS,Nakou V,Nicolai J,Nilsson M,Pall H,Peall KJ,Peters GB,Prabhakar P,Reuter MS,Rump P,Segel R,Sinnema M,Smith M,Turnpenny P,White SM,Wieczorek D,Wiethoff S,Wilson BT,Winter G,Wragg C,Pope S,Heales SJ,Morrogh D,Pittman A,Carr LJ,Perez-Dueñas B,Lin JP,Reis A,Gahl WA,Toro C,Bhatia KP,Wood NW,Kamsteeg EJ,Chong WK,Gissen P,Topf M,Dale RC,Chubb JR,Raymond FL,Kurian MA

Wed Feb 01 00:00:00 EST 2017

Abnormalities of motor function, transcription and cerebellar structure in mouse models of THAP1 dystonia.

Human molecular genetics

Ruiz M,Perez-Garcia G,Ortiz-Virumbrales M,Méneret A,Morant A,Kottwitz J,Fuchs T,Bonet J,Gonzalez-Alegre P,Hof PR,Ozelius LJ,Ehrlich ME

Sun Dec 20 00:00:00 EST 2015

Dystonia type 6 gene product Thap1: identification of a 50 kDa DNA-binding species in neuronal nuclear fractions.

Acta neuropathologica communications

Ortiz-Virumbrales M,Ruiz M,Hone E,Dolios G,Wang R,Morant A,Kottwitz J,Ozelius LJ,Gandy S,Ehrlich ME

Thu Sep 18 00:00:00 EDT 2014

Dystonia type 6 gene product Thap1: identification of a 50 kDa DNA-binding species in neuronal nuclear fractions.

Acta neuropathologica communications

Ortiz-Virumbrales M,Ruiz M,Hone E,Dolios G,Wang R,Morant A,Kottwitz J,Ozelius LJ,Gandy S,Ehrlich ME

Thu Sep 18 00:00:00 EDT 2014

Stress-induced changes in gene interactions in human cells.

Nucleic acids research

Nayak RR,Bernal WE,Lee JW,Kearns MJ,Cheung VG

Sat Feb 01 00:00:00 EST 2014

Neural expression of the transcription factor THAP1 during development in rat.

Neuroscience

Zhao Y,Xiao J,Gong S,Clara JA,Ledoux MS

Tue Feb 12 00:00:00 EST 2013

Neural expression of the transcription factor THAP1 during development in rat.

Neuroscience

Zhao Y,Xiao J,Gong S,Clara JA,Ledoux MS

Tue Feb 12 00:00:00 EST 2013

Direct interaction between causative genes of DYT1 and DYT6 primary dystonia.

Annals of neurology

Gavarini S,Cayrol C,Fuchs T,Lyons N,Ehrlich ME,Girard JP,Ozelius LJ

Fri Oct 01 00:00:00 EDT 2010

The dystonia gene DYT1 is repressed by the transcription factor THAP1 (DYT6).

Annals of neurology

Kaiser FJ,Osmanoric A,Rakovic A,Erogullari A,Uflacker N,Braunholz D,Lohnau T,Orolicki S,Albrecht M,Gillessen-Kaesbach G,Klein C,Lohmann K

Fri Oct 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: 4833431A01Rik; nuclear proapoptotic factor; THAP domain containing, apoptosis associated protein 1; THAP domain protein 1; THAP domain-containing apoptosis-associated protein 1; THAP domain-containing protein 1

Gene Aliases: 4833431A01Rik; AW490810; DYT6; THAP1

UniProt ID: (Human) Q9NVV9, (Mouse) Q8CHW1, (Rat) Q5U208

Entrez Gene ID: (Human) 55145, (Mouse) 73754, (Rat) 306547

Molecular Function: zinc finger transcription factor