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Invitrogen
TIE2 Polyclonal Antibody
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FIGURE: 1 / 1
TIE2 Antibody (PA5-47963) in IHC
Immunohistochemical analysis of TIE2 in immersion fixed whole mount zebrafish embryo (24 hours old). Samples were incubated in TIE2 polyclonal antibody (Product # PA5-47963) using a dilution of 10 µg/mL overnight at 4 °C followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue).
Product Details
PA5-47963
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Zebrafish
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant zebrafish Tie-2 Val22-His741
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Tie-2, recombinant mouse Tie-2, and rhTie-1 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells. TEK signaling appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis, and defects in TEK are associated with inherited venous malformations. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Immunoblotting showed that TIE2 expression was increased by thyroid-stimulating hormone and agents that increased intracellular cAMP. HSCs expressing the receptor tyrosine kinase TIE2 are quiescent and antiapoptotic and comprise a side population of HSCs that adhere to osteoblasts in the bone marrow niche.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Angiopoietin-1 receptor; CD202b antigen; EC 2.7.10; EC 2.7.10.1; endothelium-specific receptor tyrosine kinase 2; TEK tyrosine kinase, endothelial; TEKT1; Tektin-1; Tyrosine kinase with Ig and EGF homology domains-2; Tyrosine-protein kinase receptor Tie-2
Gene Aliases: tek; tie-2; tie2
UniProt ID: (Zebrafish) O73791
Entrez Gene ID: (Zebrafish) 30747
angiogenesis
endothelial cell proliferation
protein phosphorylation
transmembrane receptor protein tyrosine kinase signaling pathway
multicellular organism development
heart development
positive regulation of endothelial cell migration
positive regulation of phosphatidylinositol 3-kinase signaling
phosphorylation
positive regulation of kinase activity
positive regulation of MAPK cascade
positive regulation of angiogenesis
positive regulation of protein kinase B signaling
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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