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Titin Polyclonal Antibody
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FIGURE: 1 / 2
Titin Antibody (BS-9861R) in Flow
U2OS cells (black) were fixed with 4% PFA for 10 min at room temperature, permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CMD1G Antibody (bs-9861R) at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).
Product Details
BS-9861R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human Titin (Ig-like 15 domain), amino acids 2670-2703.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Monoclonal Anti-Titin can be used for study of the elastic filaments within sarcomeric structures. It is also useful as a differentiation marker in the separation of rhabdomyosarcomas from other muscle tumors. By indirect immunofluorescence the antibody displays a typical striated staining pattern on frozen sections of chicken skeletal and cardiac muscle tissues. Stains the region of the A-I junction by indirect immunofluorescence. It shows a decoration line 0.05 mm from the end of the A band in electron micro-graphs. In immunoblotting using total extracts of chicken breast muscle the antibody reacts specifically with both bands of the titin doublet, and showed no reaction with nebulin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2.7.11.1; autoimmune antigenic domain; Connectin; DKFZp451N061; FLJ26020; FLJ26409; FLJ32040; FLJ34413; FLJ39564; FLJ43066; Rhabdomyosarcoma antigen MU-RMS-40.14; titan; muscle filament; Titin; titin protein homolog; ttn; unnamed protein product
Gene Aliases: 1100001C23Rik; 2310036G12Rik; 2310057K23Rik; 2310074I15Rik; AF006999; AV006427; CMD1G; CMH9; CMPD4; CMYO5; CMYP5; D330041I19Rik; D830007G01Rik; EOMFC; HMERF; L56; LGMD2J; LGMDR10; mdm; MYLK5; SALMY; shru; TMD; TTN
UniProt ID: (Human) Q8WZ42
Entrez Gene ID: (Human) 7273, (Rat) 84015, (Mouse) 22138
nucleotide binding
protease binding
protein kinase activity
protein serine/threonine kinase activity
protein tyrosine kinase activity
structural molecule activity
calcium ion binding
protein binding
calmodulin binding
ATP binding
cytoskeletal protein binding
structural constituent of muscle
kinase activity
transferase activity
protein kinase regulator activity
enzyme binding
protein kinase binding
telethonin binding
identical protein binding
protein homodimerization activity
actinin binding
metal ion binding
actin filament binding
muscle alpha-actinin binding
structural molecule activity conferring elasticity
protein serine kinase activity
protein self-association
structural constituent of cytoskeleton
immunoglobulin receptor superfamily
skeletal muscle contraction
cardiac muscle hypertrophy
muscle contraction
striated muscle contraction
mitotic chromosome condensation
positive regulation of gene expression
protein kinase A signaling
muscle filament sliding
skeletal muscle thin filament assembly
skeletal muscle myosin thick filament assembly
detection of muscle stretch
sarcomere organization
sarcomerogenesis
positive regulation of protein secretion
response to calcium ion
cardiac myofibril assembly
cardiac muscle tissue morphogenesis
cardiac muscle cell development
cardiac muscle contraction
in utero embryonic development
heart morphogenesis
adult heart development
peptidyl-tyrosine phosphorylation
ventricular system development
forward locomotion
regulation of protein kinase activity
cardiac muscle fiber development
regulation of catalytic activity
striated muscle cell development
heart growth
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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