Immunofluorescent analysis of VIP36 showing staining in the cytoplasm of 293T cells. 293T cells were fixed in 2% paraformaldehyde/culture medium at 37°C for 30 min and stained using a VIP36 polyclonal antibody (Product # PA5-30418) diluted at 1:500. Blue: Hoechst 33343 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 58 and 273 of Human VIP36|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-30418 targets VIP36 in IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-30418 immunogen is recombinant fragment corresponding to a region within amino acids 58 and 273 of Human VIP36.
This gene encodes a type I transmembrane lectin that shuttles between the endoplasmic reticulum, the Golgi apparatus and the plasma membrane. The encoded protein binds high mannose type glycoproteins and may facilitate their sorting, trafficking and quality control.
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