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          • Primary Antibodies ›
          • WNT5A Antibodies

          Invitrogen

          WNT5A Polyclonal Antibody

          View all (24) WNT5A antibodies

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          Cite WNT5A Polyclonal Antibody

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          • Antibody Testing Data (2)
          WNT5A Antibody in Western Blot (WB)
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          WNT5A Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          WNT5A Antibody (PA5-85302) in WB

          Western blot analysis of WNT5A in whole cell lysate (30 µg). Samples were incubated with WNT5A polyclonal antibody (Product # PA5-85302) using a dilution of 1:5,000. A: Non-transfected 293T lysates; B: WNT5A transfected 293T lysates. Separated by 10% SDS-PAGE. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          WNT5A Antibody in Western Blot (WB)
          WNT5A Antibody in Western Blot (WB)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Product Details

          PA5-85302

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:10,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          Assay-dependent
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein encompassing a sequence within the center region of human WNT5A. The exact sequence is proprietary.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.36 mg/mL

          Amount

          36 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7, with 20% glycerol, 1% BSA

          Contains

          0.025% ProClin 300

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2792445

          Product Specific Information

          Keep as concentrated solution.

          Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (90%), Dog (100%), Rabbit (100%), Chicken (92%), Bovine (99%).

          Positive Control: WNT5A transfected 293T lysates, rat heart.

          Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

          Target Information

          Wnt5A is a secreted protein found in the extracellular region that belongs to the Wnt family. It is a proto-oncogene that functions in modulating cell fate and behavior during vertebrate development and has an important role in Wnt signaling pathway. It is a glycoprotein that plays an important role in cell communication and development. Wnt5A acts as a ligand for members of the frizzled family of seven transmembrane receptors. It is suspected that Wnt5A is a developmental protein. Wnt5A is a signaling molecule which affects the development of discrete regions of tissues and is likely to signal over only few cell diameters.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: epididymis secretory sperm binding protein; hWNT5A; OTTHUMP00000212837; OTTHUMP00000212861; unnamed protein product; wingless-related MMTV integration site 5A; wingless-type MMTV integration site 5A; wingless-type MMTV integration site family, member 5A; Wnt-5a; WNT-5A protein

          View more View less

          Gene Aliases: 8030457G12Rik; hWNT5A; Wnt-5a

          View more View less

          UniProt ID: (Human) P41221, (Mouse) P22725

          View more View less

          Entrez Gene ID: (Human) 7474, (Mouse) 22418, (Rat) 64566

          View more View less

          Function(s)
          receptor binding frizzled binding receptor tyrosine kinase-like orphan receptor binding cytokine activity protein binding phospholipid binding protein domain specific binding receptor agonist activity chemoattractant activity involved in axon guidance
          Process(es)
          ameboidal-type cell migration establishment of planar polarity somitogenesis positive regulation of cytokine production kidney development epithelial to mesenchymal transition neural tube closure positive regulation of protein phosphorylation positive regulation of endothelial cell proliferation heart looping morphogenesis of an epithelium positive regulation of mesenchymal cell proliferation positive regulation of cytokine production involved in immune response primary heart field specification secondary heart field specification ventricular septum development atrial septum development type B pancreatic cell development pericardium morphogenesis axis elongation protein phosphorylation inflammatory response signal transduction phospholipase C-activating G-protein coupled receptor signaling pathway Wnt signaling pathway, calcium modulating pathway JNK cascade cell-cell signaling determination of left/right symmetry axonogenesis axon guidance axonal fasciculation hindgut morphogenesis midgut development protein localization positive regulation of cell proliferation male gonad development anterior/posterior axis specification, embryo organ morphogenesis anterior/posterior pattern specification positive regulation of endothelial cell migration positive regulation of gene expression macrophage derived foam cell differentiation positive regulation of peptidyl-threonine phosphorylation positive regulation of T cell chemotaxis positive regulation of neuron projection development Wnt signaling pathway cell migration olfactory bulb interneuron development neural tube development neurogenesis positive regulation of cell-cell adhesion neuron differentiation keratinocyte differentiation lung development embryonic limb morphogenesis negative regulation of BMP signaling pathway midbrain development positive regulation of chemokine production positive regulation of interferon-gamma production positive regulation of interleukin-1 beta production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production regulation of protein localization positive regulation of peptidyl-serine phosphorylation positive regulation of Rac protein signal transduction limb morphogenesis non-canonical Wnt signaling pathway post-anal tail morphogenesis chemoattraction of serotonergic neuron axon chemorepulsion of dopaminergic neuron axon negative regulation of fibroblast growth factor receptor signaling pathway wound healing inner ear morphogenesis embryonic digit morphogenesis positive regulation of macrophage activation negative regulation of apoptotic process regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of MAPK cascade positive regulation of JUN kinase activity cell fate commitment establishment of epithelial cell apical/basal polarity negative regulation of fat cell differentiation positive regulation of protein catabolic process positive regulation of angiogenesis positive regulation of ossification positive regulation of endocytosis positive regulation of meiotic nuclear division negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade development of primary male sexual characteristics negative regulation of melanin biosynthetic process positive regulation of fibroblast proliferation paraxial mesoderm formation digestive tract morphogenesis notochord morphogenesis embryonic skeletal system development genitalia development neuron projection morphogenesis negative regulation of axon extension involved in axon guidance hypophysis morphogenesis positive regulation of epithelial cell proliferation negative regulation of epithelial cell proliferation positive regulation of inflammatory response positive regulation of dendrite morphogenesis modulation of synaptic transmission regulation of synapse organization negative chemotaxis positive regulation of small GTPase mediated signal transduction positive regulation of anagen positive regulation of protein kinase B signaling negative regulation of synapse assembly convergent extension convergent extension involved in axis elongation convergent extension involved in organogenesis uterus development cervix development vagina development canonical Wnt signaling pathway Wnt signaling pathway, planar cell polarity pathway urinary bladder development face development positive regulation of type I interferon-mediated signaling pathway lateral sprouting involved in mammary gland duct morphogenesis tube closure mesenchymal-epithelial cell signaling negative regulation of prostatic bud formation mammary gland branching involved in thelarche epithelial cell proliferation involved in mammary gland duct elongation positive regulation of response to cytokine stimulus regulation of branching involved in mammary gland duct morphogenesis mesodermal to mesenchymal transition involved in gastrulation positive regulation of macrophage cytokine production positive regulation of cartilage development somite development secondary palate development positive regulation of thymocyte apoptotic process cellular response to molecule of bacterial origin cellular response to lipopolysaccharide cellular response to calcium ion cellular response to interferon-gamma hematopoietic stem cell proliferation dopaminergic neuron differentiation cellular response to transforming growth factor beta stimulus negative regulation of mesenchymal cell proliferation primitive streak formation negative regulation of canonical Wnt signaling pathway cochlea morphogenesis melanocyte proliferation regulation of postsynaptic specialization assembly postsynaptic modulation of chemical synaptic transmission regulation of postsynapse organization regulation of postsynaptic cytosolic calcium ion concentration positive regulation of neuron projection arborization regulation of synaptic signaling by nitric oxide positive regulation of protein kinase C activity positive regulation of dendrite extension negative regulation of cell proliferation in midbrain midbrain dopaminergic neuron differentiation positive regulation of cell-cell adhesion mediated by cadherin positive regulation of non-canonical Wnt signaling pathway positive regulation of apoptotic signaling pathway lens development in camera-type eye optic cup formation involved in camera-type eye development regulation of inflammatory response cellular response to retinoic acid presynapse assembly postsynapse assembly positive regulation of protein localization to synapse excitatory synapse assembly inhibitory synapse assembly cell differentiation prostate gland development response to estradiol response to testosterone estrous cycle lung alveolus development response to glucocorticoid response to hyperoxia response to quercetin
          It has to be done as per old AB suggested Products section.

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