Invitrogen

XPD Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers
Tech Support

Product Details

PA5-28236

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 220 and 575 of Human XPD
View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2545712

Product Specific Information

Recommended positive controls: IMR32, U87-MG.

Predicted reactivity: Mouse (98%), Rat (99%), Chimpanzee (100%), Bovine (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

XPD is involved with the nucleotide excision repair pathway that is a mechanism to repair damage to DNA. The protein encoded by this gene is involved in transcription-coupled nucleotide excision repair and is an integral member of the basal transcription factor BTF2/TFIIH complex. The gene product has ATP-dependent DNA helicase activity and belongs to the RAD3/XPD subfamily of helicases. Defects in this gene can result in three different disorders, the cancer-prone syndrome xeroderma pigmentosum complementation group D, trichothiodystrophy, and Cockayne syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Basic transcription factor 2 80 kDa subunit; BTF2 p80; CXPD; DNA excision repair protein ERCC-2; DNA repair protein complementing XP-D cells; excision repair cross-complementation group 2; excision repair cross-complementing rodent repair deficiency, complementation group 2; General transcription and DNA repair factor IIH helicase subunit XPD; MGC102762; MGC126218; MGC126219; TFIIH 80 kDa subunit; TFIIH basal transcription factor complex 80 kDa subunit; TFIIH basal transcription factor complex helicase subunit; TFIIH basal transcription factor complex helicase XPB subunit; TFIIH basal transcription factor complex helicase XPD subunit; TFIIH p80; TFIIH subunit XPD; xeroderma pigmentosum complementary group D; Xeroderma pigmentosum group D-complementing protein

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Gene Aliases: AA407812; AU020867; AW240756; COFS2; CXPD; EM9; Ercc-2; ERCC2; TFIIH; TTD; TTD1; XPD; XPDC

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UniProt ID: (Human) P18074, (Mouse) O08811

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Entrez Gene ID: (Human) 2068, (Mouse) 13871

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Function(s)
DNA binding ATP-dependent DNA helicase activity protein kinase activity protein binding ATP binding protein C-terminus binding DNA-dependent ATPase activity RNA polymerase II carboxy-terminal domain kinase activity 5'-3' DNA helicase activity metal ion binding protein N-terminus binding 4 iron, 4 sulfur cluster binding nucleotide binding nucleic acid binding helicase activity ATP-dependent helicase activity hydrolase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides iron-sulfur cluster binding DNA helicase
Process(es)
nucleotide-excision repair, DNA duplex unwinding response to hypoxia in utero embryonic development transcription-coupled nucleotide-excision repair nucleotide-excision repair nucleotide-excision repair, preincision complex stabilization nucleotide-excision repair, preincision complex assembly nucleotide-excision repair, DNA incision, 3'-to lesion nucleotide-excision repair, DNA incision, 5'-to lesion transcription initiation from RNA polymerase I promoter transcription elongation from RNA polymerase I promoter termination of RNA polymerase I transcription transcription from RNA polymerase II promoter transcription initiation from RNA polymerase II promoter transcription elongation from RNA polymerase II promoter 7-methylguanosine mRNA capping protein phosphorylation apoptotic process response to oxidative stress chromosome segregation aging cell proliferation UV protection post-embryonic development viral process spinal cord development extracellular matrix organization bone mineralization central nervous system myelin formation nucleotide-excision repair, DNA incision multicellular organism growth hair cell differentiation embryonic cleavage erythrocyte maturation positive regulation of DNA binding positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter hair follicle maturation hematopoietic stem cell differentiation global genome nucleotide-excision repair regulation of mitotic cell cycle phase transition nucleobase-containing compound metabolic process DNA repair transcription, DNA-templated regulation of transcription, DNA-templated cellular response to DNA damage stimulus response to UV hair cycle process DNA duplex unwinding skin development
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