Search
Invitrogen
XPD Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 4
XPD Antibody (PA5-28236) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of XPD was performed in Mock and treated HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: XPD Polyclonal Antibody (Product # PA5-28236) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Product Details
PA5-28236
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human XPD. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: IMR32, U87-MG.
Predicted reactivity: Mouse (98%), Rat (99%), Chimpanzee (100%), Bovine (98%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
XPD is involved with the nucleotide excision repair pathway that is a mechanism to repair damage to DNA. The protein encoded by this gene is involved in transcription-coupled nucleotide excision repair and is an integral member of the basal transcription factor BTF2/TFIIH complex. The gene product has ATP-dependent DNA helicase activity and belongs to the RAD3/XPD subfamily of helicases. Defects in this gene can result in three different disorders, the cancer-prone syndrome xeroderma pigmentosum complementation group D, trichothiodystrophy, and Cockayne syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Basic transcription factor 2 80 kDa subunit; BTF2 p80; CXPD; DNA 5'-3' helicase XPD; DNA excision repair protein ERCC-2; DNA repair protein complementing XP-D cells; ERCC2; excision repair cross-complementation group 2; excision repair cross-complementing rodent repair deficiency, complementation group 2; General transcription and DNA repair factor IIH helicase subunit XPD; MGC102762; MGC126218; MGC126219; TFIIH 80 kDa subunit; TFIIH basal transcription factor complex 80 kDa subunit; TFIIH basal transcription factor complex helicase subunit; TFIIH basal transcription factor complex helicase XPD subunit; TFIIH p80; TFIIH subunit XPD; unnamed protein product; xeroderma pigmentosum complementary group D; Xeroderma pigmentosum group D-complementing protein
Gene Aliases: AA407812; AU020867; AW240756; COFS2; CXPD; EM9; Ercc-2; ERCC2; TFIIH; TTD; TTD1; XPD; XPDC
UniProt ID: (Human) P18074, (Mouse) O08811
Entrez Gene ID: (Human) 2068, (Mouse) 13871
nucleotide binding
nucleic acid binding
DNA binding
DNA helicase activity
damaged DNA binding
helicase activity
protein binding
ATP binding
hydrolase activity
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
isomerase activity
ATPase activity
protein binding, bridging
5'-3' DNA helicase activity
metal ion binding
iron-sulfur cluster binding
4 iron, 4 sulfur cluster binding
ATP-dependent DNA helicase activity
protein kinase activity
protein C-terminus binding
ATP-dependent helicase activity
DNA-dependent ATPase activity
RNA polymerase II carboxy-terminal domain kinase activity
protein N-terminus binding
DNA helicase
maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
response to hypoxia
in utero embryonic development
nucleobase-containing compound metabolic process
DNA repair
transcription-coupled nucleotide-excision repair
nucleotide-excision repair
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription elongation from RNA polymerase I promoter
transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
apoptotic process
cellular response to DNA damage stimulus
response to oxidative stress
chromosome segregation
determination of adult lifespan
response to UV
UV protection
post-embryonic development
spinal cord development
hair cycle process
extracellular matrix organization
bone mineralization
central nervous system myelin formation
multicellular organism growth
hair cell differentiation
embryonic cleavage
ribosomal small subunit biogenesis
erythrocyte maturation
skin development
positive regulation of mitotic recombination
insulin-like growth factor receptor signaling pathway
embryonic organ development
hair follicle maturation
hematopoietic stem cell differentiation
hematopoietic stem cell proliferation
intrinsic apoptotic signaling pathway by p53 class mediator
regulation of mitotic cell cycle phase transition
regulation of transcription, DNA-templated
protein phosphorylation
aging
cell proliferation
DNA duplex unwinding
nucleotide-excision repair, DNA incision
positive regulation of DNA binding
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support