Proteintech

ZEB1 Polyclonal Antibody

Product Image Gallery

ZEB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 21544-1-AP ( ZEB1 Antibody) at dilution of 1:500 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

ZEB1 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 21544-1-AP (ZEB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

ZEB1 Antibody in Immunoprecipitation (IP) — IP result of anti-ZEB1 (IP:21544-1-AP, 4ug; Detection:21544-1-AP 1:500) with MCF-7 cells lysate 4000ug.

Product Details

Applications

Tested Dilution

Publications

ChIP assay (ChIP)

Assay-dependent

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunoprecipitation (IP)

0.5-4.0 µg

Western Blot (WB)

1:500-1:2400

View 25 publications 25 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ZEB1 Fusion Protein Ag16204

Conjugate

Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

The calculated molecular weight of ZEB1 is 124 kDa, but the post phosphorylation of protein is about 190-210 kDa. This is a rabbit polyclonal antibody raised against part of the full length of ZEB1 of human.

Target Information

ZEB1 (Zinc finger E-box-binding homeobox 1) acts as a transcriptional repressor. It inhibits interleukin-2 (IL-2) gene expression. ZEB1 enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and depending on the cell type. It represses E-cadherin promoters and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. ZEB1 also represses BCL6 transcription in the presence of the corepressor CTBP1. It postively regulates neuronal differentiation and represses RCOR1 transcription activation during neurogenesis. Mutations in the gene can result in corneal dystrophy, posterior polymorphous and corneal dystrophy, Fuchs endothelial 6.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Delta EF1; delta-crystallin enhancer binding factor 1; DELTA-EF1; MEB1; MGC133261; Negative regulator of IL2; NIL-2-A; NIL-2-A zinc finger protein; NIL-2A; OTTHUMP00000019405; posterior polymorphous corneal dystrophy 3; RP11-472N13.4; TCF-8; Transcription factor 8; transcription factor 8 (represses interleukin 2 expression); twirler; Zfhep; Zinc finger E-box-binding homeobox 1; Zinc finger homeobox protein 1a; Zinc finger homeodomain enhancer-binding protein

Gene Aliases: 3110032K11Rik; [delta]EF1; AREB6; BZP; DELTAEF1; FECD6; MEB1; Nil2; NIL2A; PPCD3; TCF-8; Tcf18; TCF8; Tw; ZEB; ZEB1; ZFHEP; ZFHX1A; Zfx1a; Zfx1ha

UniProt ID: (Human) P37275, (Mouse) Q64318, (Rat) Q62947

Entrez Gene ID: (Human) 6935, (Mouse) 21417, (Rat) 25705

Molecular Function: KRAB box transcription factor transcription factor zinc finger transcription factor