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ZEB1 Polyclonal Antibody

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ZEB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 21544-1-AP ( ZEB1 Antibody) at dilution of 1:500 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

ZEB1 Antibody in Western Blot (WB) — PC-3 cells were subjected to SDS PAGE followed by western blot with 21544-1-AP (ZEB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

ZEB1 Antibody in Immunoprecipitation (IP) — IP result of anti-ZEB1 (IP:21544-1-AP, 4ug; Detection:21544-1-AP 1:500) with MCF-7 cells lysate 4000ug.

Product Details

21544-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2400	View 25 publications 25 publications
Immunohistochemistry (IHC)	-	View 3 publications 3 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunoprecipitation (IP)	0.5-4.0 µg	-
ChIP assay (ChIP)	Assay-dependent	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ZEB1 Fusion Protein Ag16204 (391-740 aa encoded by BC112392) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.27 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

The calculated molecular weight of ZEB1 is 124 kDa, but the post phosphorylation of protein is about 190-210 kDa. This is a rabbit polyclonal antibody raised against part of the full length of ZEB1 of human.

Immunogen sequence: MVQAVVLPT VGLVSPISIN LSDIQNVLKV AVDGNVIRQV LENNQANLAS KEQETINASP IQQGGHSVIS AISLPLVDQD GTTKIIINYS LEQPSQLQVV PQNLKKENPV ATNSCKSEKL PEDLTVKSEK DKSFEGGVND STCLLCDDCP GDINALPELK HYDLKQPTQP PPLPAAEAEK PESSVSSATG DGNLSPSQPP LKNLLSLLKA YYALNAQPSA EELSKIADSV NLPLDVVKKW FEKMQAGQIS VQSSEPSSPE PGKVNIPAKN NDQPQSANAN EPQDSTVNLQ SPLKMTNSPV LPVGSTTNGS RSSTPSPSPL NLSSSRNTQG YLYTAEGAQE EPQVEPLDLS L (391-740 aa encoded by BC112392)

Target Information

ZEB1 (Zinc finger E-box-binding homeobox 1) acts as a transcriptional repressor. It inhibits interleukin-2 (IL-2) gene expression. ZEB1 enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and depending on the cell type. It represses E-cadherin promoters and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. ZEB1 also represses BCL6 transcription in the presence of the corepressor CTBP1. It postively regulates neuronal differentiation and represses RCOR1 transcription activation during neurogenesis. Mutations in the gene can result in corneal dystrophy, posterior polymorphous and corneal dystrophy, Fuchs endothelial 6.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Downregulation of DAPK1 promotes the stemness of cancer stem cells and EMT process by activating ZEB1 in colorectal cancer.

Journal of molecular medicine (Berlin, Germany)

Yuan W,Ji J,Shu Y,Chen J,Liu S,Wu L,Zhou Z,Liu Z,Tang Q,Zhang X,Shu X

Tue Jan 01 00:00:00 EST 2019

Myostatin promotes the epithelial-to-mesenchymal transition of the dermomyotome during somitogenesis.

Developmental dynamics : an official publication of the American Association of Anatomists

Zhou Y,Zhang Y,Zhu D

Sat Dec 01 00:00:00 EST 2018

miR-26a/HOXC9 Dysregulation Promotes Metastasis and Stem Cell-Like Phenotype of Gastric Cancer.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Peng X,Kang Q,Wan R,Wang Z

Tue Oct 16 00:00:00 EDT 2018

YAP drives cutaneous squamous cell carcinoma formation and progression.

eLife

Vincent-Mistiaen Z,Elbediwy A,Vanyai H,Cotton J,Stamp G,Nye E,Spencer-Dene B,Thomas GJ,Mao J,Thompson B

Thu Sep 20 00:00:00 EDT 2018

The effects of ultrasound exposure on P-glycoprotein-mediated multidrug resistance in vitro and in vivo.

Journal of experimental & clinical cancer research : CR

Huang C,Huang S,Li H,Li X,Li B,Zhong L,Wang J,Zou M,He X,Zheng H,Si X,Liao W,Liao Y,Yang L,Bin J

Wed Sep 19 00:00:00 EDT 2018

SNORA42 enhances prostate cancer cell viability, migration and EMT and is correlated with prostate cancer poor prognosis.

The international journal of biochemistry & cell biology

Yi C,Wan X,Zhang Y,Fu F,Zhao C,Qin R,Wu H,Li Y,Huang Y

Sat Sep 01 00:00:00 EDT 2018

LncRNA UCA1 sponges miR-204-5p to promote migration, invasion and epithelial-mesenchymal transition of glioma cells via upregulation of ZEB1.

Pathology, research and practice

Liang C,Yang Y,Guan J,Lv T,Qu S,Fu Q,Zhao H

Sat Sep 01 00:00:00 EDT 2018

Long noncoding RNA NEAT1, regulated by LIN28B, promotes cell proliferation and migration through sponging miR-506 in high-grade serous ovarian cancer.

Cell death & disease

Yong W,Yu D,Jun Z,Yachen D,Weiwei W,Midie X,Xingzhu J,Xiaohua W

Tue Aug 28 00:00:00 EDT 2018

Gene Correction Reverses Ciliopathy and Photoreceptor Loss in iPSC-Derived Retinal Organoids from Retinitis Pigmentosa Patients.

Stem cell reports

Deng WL,Gao ML,Lei XL,Lv JN,Zhao H,He KW,Xia XX,Li LY,Chen YC,Li YP,Pan D,Xue T,Jin ZB

Tue Apr 10 00:00:00 EDT 2018

MicroRNA-140 Inhibits the Epithelial-Mesenchymal Transition and Metastasis in Colorectal Cancer.

Molecular therapy. Nucleic acids

Li J,Zou K,Yu L,Zhao W,Lu Y,Mao J,Wang B,Wang L,Fan S,Song B,Li L

Fri Mar 02 00:00:00 EST 2018

PIM1 mediates epithelial-mesenchymal transition by targeting Smads and c-Myc in the nucleus and potentiates clear-cell renal-cell carcinoma oncogenesis.

Cell death & disease

Zhao B,Liu L,Mao J,Zhang Z,Wang Q,Li Q

Thu Feb 22 00:00:00 EST 2018

MiR-200c Inhibits the Tumor Progression of Glioma via Targeting Moesin.

Theranostics

Qin Y,Chen W,Liu B,Zhou L,Deng L,Niu W,Bao D,Cheng C,Li D,Liu S,Niu C

Mon Feb 12 00:00:00 EST 2018

ZEB1 stimulates breast cancer growth by up-regulating hTERT expression.

Biochemical and biophysical research communications

Yu P,Shen X,Yang W,Zhang Y,Liu C,Huang T

Mon Jan 22 00:00:00 EST 2018

ZEB1 stimulates breast cancer growth by up-regulating hTERT expression.

Biochemical and biophysical research communications

Yu P,Shen X,Yang W,Zhang Y,Liu C,Huang T

Mon Jan 22 00:00:00 EST 2018

ADAM12 induces EMT and promotes cell migration, invasion and proliferation in pituitary adenomas via EGFR/ERK signaling pathway.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Wang J,Zhang Z,Li R,Mao F,Sun W,Chen J,Zhang H,Bartsch JW,Shu K,Lei T

Mon Jan 01 00:00:00 EST 2018

Core 3 mucin-type O-glycan restoration in colorectal cancer cells promotes MUC1/p53/miR-200c-dependent epithelial identity.

Oncogene

Ye J,Wei X,Shang Y,Pan Q,Yang M,Tian Y,He Y,Peng Z,Chen L,Chen W,Wang R

Thu Nov 16 00:00:00 EST 2017

HIF-1α/Ascl2/miR-200b regulatory feedback circuit modulated the epithelial-mesenchymal transition (EMT) in colorectal cancer cells.

Experimental cell research

Shang Y,Chen H,Ye J,Wei X,Liu S,Wang R

Wed Nov 15 00:00:00 EST 2017

miR-200c regulates endothelin-1 induced PASMCs abnormal proliferation and apoptosis.

IUBMB life

Yuan C,Xu M,Rong R,Mei Y,Cai W,Li L,Xue Y,Zhu B,Sun K,Han L

Wed Nov 01 00:00:00 EDT 2017

A non-canonical pathway regulates ER stress signaling and blocks ER stress-induced apoptosis and heart failure.

Nature communications

Yao Y,Lu Q,Hu Z,Yu Y,Chen Q,Wang QK

Tue Jul 25 00:00:00 EDT 2017

Linc-DYNC2H1-4 promotes EMT and CSC phenotypes by acting as a sponge of miR-145 in pancreatic cancer cells.

Cell death & disease

Gao Y,Zhang Z,Li K,Gong L,Yang Q,Huang X,Hong C,Ding M,Yang H

Thu Jul 13 00:00:00 EDT 2017

Disrupting MALAT1/miR-200c sponge decreases invasion and migration in endometrioid endometrial carcinoma.

Cancer letters

Li Q,Zhang C,Chen R,Xiong H,Qiu F,Liu S,Zhang M,Wang F,Wang Y,Zhou X,Xiao G,Wang X,Jiang Q

Thu Dec 01 00:00:00 EST 2016

Tanshinone IIA attenuates epithelial-mesenchymal transition to inhibit the tracheal narrowing.

The Journal of surgical research

Duan H,Ma L,Liu H,Zhang Y,Zhang Z,Yan X,Li X

Tue Nov 01 00:00:00 EDT 2016

Zeb1 Is a Potential Regulator of Six2 in the Proliferation, Apoptosis and Migration of Metanephric Mesenchyme Cells.

International journal of molecular sciences

Gu Y,Zhao Y,Zhou Y,Xie Y,Ju P,Long Y,Liu J,Ni D,Cao F,Lyu Z,Mao Z,Hao J,Li Y,Wan Q,Kanyomse Q,Liu Y,Ren D,Ning Y,Li X,Zhou Q,Li B

Sat Aug 06 00:00:00 EDT 2016

Conditional ablation of TGF-β signaling inhibits tumor progression and invasion in an induced mouse bladder cancer model.

Scientific reports

Liang Y,Zhu F,Zhang H,Chen D,Zhang X,Gao Q,Li Y

Tue Jul 05 00:00:00 EDT 2016

Downregulation of ACE2/Ang-(1-7)/Mas axis promotes breast cancer metastasis by enhancing store-operated calcium entry.

Cancer letters

Yu C,Tang W,Wang Y,Shen Q,Wang B,Cai C,Meng X,Zou F

Fri Jul 01 00:00:00 EDT 2016

Attenuated LKB1-SIK1 signaling promotes epithelial-mesenchymal transition and radioresistance of non-small cell lung cancer cells.

Chinese journal of cancer

Yao YH,Cui Y,Qiu XN,Zhang LZ,Zhang W,Li H,Yu JM

Tue Jun 07 00:00:00 EDT 2016

PFN2, a novel marker of unfavorable prognosis, is a potential therapeutic target involved in esophageal squamous cell carcinoma.

Journal of translational medicine

Cui XB,Zhang SM,Xu YX,Dang HW,Liu CX,Wang LH,Yang L,Hu JM,Liang WH,Jiang JF,Li N,Li Y,Chen YZ,Li F

Tue May 17 00:00:00 EDT 2016

MEP1A contributes to tumor progression and predicts poor clinical outcome in human hepatocellular carcinoma.

Hepatology (Baltimore, Md.)

OuYang HY,Xu J,Luo J,Zou RH,Chen K,Le Y,Zhang YF,Wei W,Guo RP,Shi M

Fri Apr 01 00:00:00 EDT 2016

TGFβ1 secreted by cancer-associated fibroblasts induces epithelial-mesenchymal transition of bladder cancer cells through lncRNA-ZEB2NAT.

Scientific reports

Zhuang J,Lu Q,Shen B,Huang X,Shen L,Zheng X,Huang R,Yan J,Guo H

Wed Jul 08 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: Delta EF1; delta-crystallin enhancer binding factor 1; DELTA-EF1; MEB1; MGC133261; Negative regulator of IL2; NIL-2-A; NIL-2-A zinc finger protein; NIL-2A; OTTHUMP00000019405; posterior polymorphous corneal dystrophy 3; RP11-472N13.4; TCF-8; Transcription factor 8; transcription factor 8 (represses interleukin 2 expression); twirler; Zfhep; Zinc finger E-box-binding homeobox 1; Zinc finger homeobox protein 1a; Zinc finger homeodomain enhancer-binding protein

Gene Aliases: 3110032K11Rik; [delta]EF1; AREB6; BZP; DELTAEF1; FECD6; MEB1; Nil2; NIL2A; PPCD3; TCF-8; Tcf18; TCF8; Tw; ZEB; ZEB1; ZFHEP; ZFHX1A; Zfx1a; Zfx1ha

UniProt ID: (Human) P37275, (Mouse) Q64318, (Rat) Q62947

Entrez Gene ID: (Human) 6935, (Mouse) 21417, (Rat) 25705

Molecular Function: C2H2 zinc finger transcription factor