Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500-1:2400 | View 25 publications 25 publications |
Immunohistochemistry (IHC) |
- | View 3 publications 3 publications |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:50-1:500 | - |
Immunoprecipitation (IP) |
0.5-4.0 µg | - |
ChIP assay (ChIP) |
Assay-dependent | View 1 publication 1 publication |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse, Rat |
Published species |
Human, Mouse, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
ZEB1 Fusion Protein Ag16204 (391-740 aa encoded by BC112392) |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.27 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20°C |
The calculated molecular weight of ZEB1 is 124 kDa, but the post phosphorylation of protein is about 190-210 kDa. This is a rabbit polyclonal antibody raised against part of the full length of ZEB1 of human.
Immunogen sequence: MVQAVVLPT VGLVSPISIN LSDIQNVLKV AVDGNVIRQV LENNQANLAS KEQETINASP IQQGGHSVIS AISLPLVDQD GTTKIIINYS LEQPSQLQVV PQNLKKENPV ATNSCKSEKL PEDLTVKSEK DKSFEGGVND STCLLCDDCP GDINALPELK HYDLKQPTQP PPLPAAEAEK PESSVSSATG DGNLSPSQPP LKNLLSLLKA YYALNAQPSA EELSKIADSV NLPLDVVKKW FEKMQAGQIS VQSSEPSSPE PGKVNIPAKN NDQPQSANAN EPQDSTVNLQ SPLKMTNSPV LPVGSTTNGS RSSTPSPSPL NLSSSRNTQG YLYTAEGAQE EPQVEPLDLS L (391-740 aa encoded by BC112392)
ZEB1 (Zinc finger E-box-binding homeobox 1) acts as a transcriptional repressor. It inhibits interleukin-2 (IL-2) gene expression. ZEB1 enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and depending on the cell type. It represses E-cadherin promoters and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. ZEB1 also represses BCL6 transcription in the presence of the corepressor CTBP1. It postively regulates neuronal differentiation and represses RCOR1 transcription activation during neurogenesis. Mutations in the gene can result in corneal dystrophy, posterior polymorphous and corneal dystrophy, Fuchs endothelial 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Delta EF1; delta-crystallin enhancer binding factor 1; DELTA-EF1; MEB1; MGC133261; Negative regulator of IL2; NIL-2-A; NIL-2-A zinc finger protein; NIL-2A; OTTHUMP00000019405; posterior polymorphous corneal dystrophy 3; RP11-472N13.4; TCF-8; Transcription factor 8; transcription factor 8 (represses interleukin 2 expression); twirler; Zfhep; Zinc finger E-box-binding homeobox 1; Zinc finger homeobox protein 1a; Zinc finger homeodomain enhancer-binding protein
Gene Aliases: 3110032K11Rik; [delta]EF1; AREB6; BZP; DELTAEF1; FECD6; MEB1; Nil2; NIL2A; PPCD3; TCF-8; Tcf18; TCF8; Tw; ZEB; ZEB1; ZFHEP; ZFHX1A; Zfx1a; Zfx1ha
UniProt ID: (Human) P37275, (Mouse) Q64318, (Rat) Q62947
Entrez Gene ID: (Human) 6935, (Mouse) 21417, (Rat) 25705
Molecular Function:
C2H2 zinc finger transcription factor
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