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Invitrogen
ZNF554 Polyclonal Antibody
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FIGURE: 1 / 2
ZNF554 Antibody (PA5-30907) in ICC/IF
ZNF554 Polyclonal Antibody detects ZNF554 protein at nucleus by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZNF554 protein stained by ZNF554 Polyclonal Antibody (Product # PA5-30907) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Product Details
PA5-30907
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human ZNF554. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: PC-3.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ZNF554, or Zinc Finger Protein 554, is a member of the Kruppel-associated box domain-zinc finger proteins (KRAB-ZNF) subfamily, residing on chromosome 19p13. This region is characterized by a concentration of heterochromatin proteins, which indicates tightly regulated gene expression. ZNF554 is implicated in various cellular processes including transcription regulation and apoptosis. Dysregulation of ZNF554 has been linked to several diseases; notably, its decreased expression is associated with poor prognosis in gliomas, specifically grades III and IV astrocytomas, where it affects the PI3K-Akt signaling pathway. It also plays a role in placental development, with dysfunction contributing to conditions such as fetal growth restriction (FGR). Additionally, ZNF554 transcription is influenced by factors like ZEB1, ZEB2, and hypoxic conditions, with neighboring hypoxia-response elements showing hypermethylation in preeclampsia. This gene's expression can significantly alter the transcriptional landscape, with overexpression leading to the differential regulation of hundreds of genes, underscoring its importance in cellular regulation.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ34817; Zinc finger protein 554
Gene Aliases: ZNF554
UniProt ID: (Human) Q86TJ5
Entrez Gene ID: (Human) 115196
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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