Immunofluorescent analysis of ADR-Beta-1 (green) in untreated HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. Cells were then blocked with 5% normal goat serum (Product #31873) for 15 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing ADR-Beta-1 (Product # PA1-049), at a dilution of 1:100 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-rabbit secondary antibody (Product# 35552) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei were stained with Hoechst 33342 dye (Product# 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Rat, Cat, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues H(394) G D R P R A S G C L A R A G(408) of mouse B1AR.|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-049 detects beta-1 adrenergic receptor (B1AR) from , human, mouse, non-human primate, and rat tissues.
PA1-049 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~50.5 kDa band representing B1AR in mouse kidney membrane preparation. Immunofluorescence staining of B1AR in mouse kidney distal tubule with PA1-049 yields a pattern consistent with plasma membrane staining.
The PA1-049 immunogen is a synthetic peptide corresponding to residues H(394) G D R P R A S G C L A R A G(408) of mouse B1AR. This sequence is completely conserved between mouse and rat B1AR.
Adrenergic receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily that bind the endogenous catecholamines epinephrine and norepinephrine. Pharmacological, structural, and molecular cloning data indicate significant heterogeneity within this receptor family. Nine receptor subtypes have been identified thus far including three alpha-1 AR subtypes (1A/D, 1B, and 1C), three alpha-2 ARs (2A, 2B, and 2C), and three beta AR subtypes (1, 2, and 3). ARs participate in either the onset or maintenance of several disease states including hypertension, cardiac dysfunction (congestive heart failure, ischemia, arrhythmias), diabetes, glaucoma, depression, and impotence. BARs participate in diverse processes including development, behavior, cardiac function, smooth muscle tone, and metabolism. In gene-knockout experiments, the majority of mice that lack the B1AR gene die prenatally and those that do survive until adulthood display abnormal cardiac function. Other studies have shown that the direct regulation of cardiac B1AR density by thyroid hormones occurs at the transcriptional level and is modulated by the catecholamine sensitive-adenylyl cyclase system. Evidence for the effects of cAMP on B1AR regulation has come from a study of members of the cAMP response element (CRE) modulator (CREM) family of transcription factors in transformed cell lines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Upregulation of ß1-adrenoceptors is involved in the formation of gastric dysmotility in the 6-hydroxydopamine rat model of Parkinson's disease.
PA1-049 was used in immunohistochemistry and western blot to study gastric paresis in a rat model of Parkinson's disease and the the role played by increased levels of beta1-adrenoceptors
|Song J,Zheng L,Zhang X,Feng X,Fan R,Sun L,Hong F,Zhang Y,Zhu J||Translational research : the journal of laboratory and clinical medicine (164:22)||2014|
Infusion of the beta-adrenergic blocker esmolol attenuates myocardial dysfunction in septic rats.
PA1-049 was used in immunohistochemistry to investigate the effect of the beta-adrenergic blocker esmolol on myocardial dysfunction in septic rats
|Suzuki T,Morisaki H,Serita R,Yamamoto M,Kotake Y,Ishizaka A,Takeda J||Critical care medicine (33:2294)||2005|
Effects of monocular deprivation on the expression pattern of alpha-1 and beta-1 adrenergic receptors in the kitten visual cortex.
PA1-049 was used in immunohistochemistry to study the regulation of adrenergic receptors by sensory afferents in kitten brains
|Nakadate K,Imamura K,Watanabe Y||Neuroscience research (40:155)||2001|
Heterogeneous ventricular sympathetic innervation, altered beta-adrenergic receptor expression, and rhythm instability in mice lacking the p75 neurotrophin receptor.
PA1-049 was used in western blot to study the role of p75 neurotrophin receptor in innervation and rhythm stability in mice
|Lorentz CU,Alston EN,Belcik T,Lindner JR,Giraud GD,Habecker BA||American journal of physiology. Heart and circulatory physiology (298:H1652)||2010|
Norepinephrine transporter is involved in down-regulation of beta1-adrenergic receptors caused by adjuvant arthritis.
PA1-049 was used in western blot to investigate the effects of inflammation on beta1-adrenergic receptors and norepinephrine transporter (NET) density
|Clements JD,Jamali F||Journal of pharmacy and pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques (12:337)||2010|
Cardioprotective effects of exercise training on myofilament calcium activation in ovariectomized rats.
PA1-049 was used in western blot to study whether the cardioprotective effects of exercise training affects calcium activation in cardiac myofilament in ovariectomized rats
|Bupha-Intr T,Wattanapermpool J||Journal of applied physiology (Bethesda, Md. : 1985) (96:1755)||2004|
Adrenergic receptor agonists prevent bile duct injury induced by adrenergic denervation by increased cAMP levels and activation of Akt.
PA1-049 was used in immunocytochemistry to study how cholangiocyte proliferative and secretory responses to bile duct ligation are influenced by adrenergic innervation
|Glaser S,Alvaro D,Francis H,Ueno Y,Marucci L,Benedetti A,De Morrow S,Marzioni M,Mancino MG,Phinizy JL,Reichenbach R,Fava G,Summers R,Venter J,Alpini G||American journal of physiology. Gastrointestinal and liver physiology (290:G813)||2006|