Invitrogen

beta-1 Adrenergic Receptor Polyclonal Antibody

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beta-1 Adrenergic Receptor Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ADR-Beta-1 (green) in untreated HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. Cells were then blocked with 5% normal goat serum (Product # 31873) for 15 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing ADR-Beta-1 (Product # PA1-049), at a dilution of 1:100 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-rabbit secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.

beta-1 Adrenergic Receptor Antibody in Immunohistochemistry (IHC) — Immunolocalization of B1AR in mouse kidney distal tubule using Product # PA1-049.

beta-1 Adrenergic Receptor Antibody in Western Blot (WB) — Western blot analysis of ADR-Beta-1 was performed by loading 25 µg of various whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a rabbit polyclonal antibody recognizing ADR-Beta-1 (Product # PA1-049) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody (Product # 31460) at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Pierce Super Signal West Pico (Product # 34077).

Product Details

PA1-049

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	View 8 publications 8 publications
Immunohistochemistry (IHC)	Assay-dependent	View 5 publications 5 publications
Immunocytochemistry (ICC/IF)	1:100	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human, Mouse, Non-human primate, Rat
Published species	Cat, Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide corresponding to residues H(394) G D R P R A S G C L A R A G(408) of mouse B1AR. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	Conc. Not Determined
Storage buffer	whole serum
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2289444

Product Specific Information

PA1-049 detects beta-1 adrenergic receptor (B1AR) from , human, mouse, non-human primate, and rat tissues.

PA1-049 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~50.5 kDa band representing B1AR in mouse kidney membrane preparation. Immunofluorescence staining of B1AR in mouse kidney distal tubule with PA1-049 yields a pattern consistent with plasma membrane staining.

The PA1-049 immunogen is a synthetic peptide corresponding to residues H(394) G D R P R A S G C L A R A G(408) of mouse B1AR. This sequence is completely conserved between mouse and rat B1AR.

Target Information

Adrenergic receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily that bind the endogenous catecholamines epinephrine and norepinephrine. Pharmacological, structural, and molecular cloning data indicate significant heterogeneity within this receptor family. Nine receptor subtypes have been identified thus far including three alpha-1 AR subtypes (1A/D, 1B, and 1C), three alpha-2 ARs (2A, 2B, and 2C), and three beta AR subtypes (1, 2, and 3). ARs participate in either the onset or maintenance of several disease states including hypertension, cardiac dysfunction (congestive heart failure, ischemia, arrhythmias), diabetes, glaucoma, depression, and impotence. BARs participate in diverse processes including development, behavior, cardiac function, smooth muscle tone, and metabolism. In gene-knockout experiments, the majority of mice that lack the B1AR gene die prenatally and those that do survive until adulthood display abnormal cardiac function. Other studies have shown that the direct regulation of cardiac B1AR density by thyroid hormones occurs at the transcriptional level and is modulated by the catecholamine sensitive-adenylyl cyclase system. Evidence for the effects of cAMP on B1AR regulation has come from a study of members of the cAMP response element (CRE) modulator (CREM) family of transcription factors in transformed cell lines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Positive Effects of a Mediterranean Diet Supplemented with Almonds on Female Adipose Tissue Biology in Severe Obesity.

Nutrients

Osorio-Conles Ó,Olbeyra R,Moizé V,Ibarzabal A,Giró O,Viaplana J,Jiménez A,Vidal J,de Hollanda A

PA1-049 was used in Immunohistochemistry to support a protective effect of a Mediterranean diet supplemented with almonds on obesity-related WAT dysfunction.

Fri Jun 24 00:00:00 EDT 2022

Deletion of adipose triglyceride lipase abolishes blood flow increase after β3-adrenergic stimulation in visceral adipose tissue of mice.

The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology

Lee HJ,Jin BY,Park MR,Seo KS,Jeong YT,Choi SH,Kim DH

PA1-049 was used in Immunohistochemistry to identify the role of each β-AR subtype in the regulation of ATBF in mice.

Thu Jul 01 00:00:00 EDT 2021

Importance of the renal ion channel TRPM6 in the circadian secretion of renin to raise blood pressure.

Nature communications

Funato Y,Yamazaki D,Okuzaki D,Yamamoto N,Miki H

PA1-049 was used in Western Blot to indicate crucial roles of TRPM6 in the circadian regulation of blood pressure.

Thu Jun 17 00:00:00 EDT 2021

Aldosterone Jeopardizes Myocardial Insulin and β-Adrenergic Receptor Signaling via G Protein-Coupled Receptor Kinase 2.

Frontiers in pharmacology

Cannavo A,Marzano F,Elia A,Liccardo D,Bencivenga L,Gambino G,Perna C,Rapacciuolo A,Cittadini A,Ferrara N,Paolocci N,Koch WJ,Rengo G

PA1-049 was used in Western Blotting to reveal that GRK2 activity is a critical player downstream of the aldosterone signaling pathway; therefore, inhibiting this kinase is an attractive strategy to prevent the cardiac structural disarray and dysfunction that accompany any clinical condition accompanied by hyperaldosteronism.

Tue Sep 29 00:00:00 EDT 2020

Cardiomyocyte slowly activating delayed rectifier potassium channel: regulation by exercise and β-adrenergic signaling.

Journal of applied physiology (Bethesda, Md. : 1985)

Wang X,Fitts RH

PA1-049 was used in Western Blotting to elucidate the mechanisms by which exercise training alters β-adrenergic regulation and to determine the involvement of delayed rectifier potassium channels (IKr and IKs) in the response.

Fri May 01 00:00:00 EDT 2020

Noradrenaline Sensitivity Is Severely Impaired in Immortalized Adipose-Derived Mesenchymal Stem Cell Line.

International journal of molecular sciences

Tyurin-Kuzmin PA,Chechekhin VI,Ivanova AM,Dyikanov DT,Sysoeva VY,Kalinina NI,Tkachuk VA

PA1-049 was used in Immunocytochemistry-immunoflourescence to study the usage of immortalised cells for multipotent stem/stromal cell-based regenerative medicine.

Thu Nov 22 00:00:00 EST 2018

Atrial arrhythmias and autonomic dysfunction in rats exposed to chronic intermittent hypoxia.

American journal of physiology. Heart and circulatory physiology

Bober SL,Ciriello J,Jones DL

PA1-049 was used in Western Blotting to study the effect of chronic intermittent hypoxia on atrial electrophysiology in atrial fibrillation.

Fri Jun 01 00:00:00 EDT 2018

Upregulation of β1-adrenoceptors is involved in the formation of gastric dysmotility in the 6-hydroxydopamine rat model of Parkinson's disease.

Translational research : the journal of laboratory and clinical medicine

Song J,Zheng L,Zhang X,Feng X,Fan R,Sun L,Hong F,Zhang Y,Zhu J

PA1-049 was used in immunohistochemistry and western blot to study gastric paresis in a rat model of Parkinson's disease and the the role played by increased levels of beta1-adrenoceptors

Tue Jul 01 00:00:00 EDT 2014

Regulation of nicotinic acetylcholine receptor turnover by MuRF1 connects muscle activity to endo/lysosomal and atrophy pathways.

Age (Dordrecht, Netherlands)

Rudolf R,Bogomolovas J,Strack S,Choi KR,Khan MM,Wagner A,Brohm K,Hanashima A,Gasch A,Labeit D,Labeit S

Published figure using beta-1 Adrenergic Receptor polyclonal antibody (Product # PA1-049) in Western Blot

Tue Oct 01 00:00:00 EDT 2013

Heterogeneous ventricular sympathetic innervation, altered beta-adrenergic receptor expression, and rhythm instability in mice lacking the p75 neurotrophin receptor.

American journal of physiology. Heart and circulatory physiology

Lorentz CU,Alston EN,Belcik T,Lindner JR,Giraud GD,Habecker BA

PA1-049 was used in western blot to study the role of p75 neurotrophin receptor in innervation and rhythm stability in mice

Tue Jun 01 00:00:00 EDT 2010

Norepinephrine transporter is involved in down-regulation of beta1-adrenergic receptors caused by adjuvant arthritis.

Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques

Clements JD,Jamali F

PA1-049 was used in western blot to investigate the effects of inflammation on beta1-adrenergic receptors and norepinephrine transporter (NET) density

Fri Apr 02 00:00:00 EDT 2010

Adrenergic receptor agonists prevent bile duct injury induced by adrenergic denervation by increased cAMP levels and activation of Akt.

American journal of physiology. Gastrointestinal and liver physiology

Glaser S,Alvaro D,Francis H,Ueno Y,Marucci L,Benedetti A,De Morrow S,Marzioni M,Mancino MG,Phinizy JL,Reichenbach R,Fava G,Summers R,Venter J,Alpini G

PA1-049 was used in immunocytochemistry to study how cholangiocyte proliferative and secretory responses to bile duct ligation are influenced by adrenergic innervation

Sat Apr 01 00:00:00 EST 2006

Infusion of the beta-adrenergic blocker esmolol attenuates myocardial dysfunction in septic rats.

Critical care medicine

Suzuki T,Morisaki H,Serita R,Yamamoto M,Kotake Y,Ishizaka A,Takeda J

PA1-049 was used in immunohistochemistry to investigate the effect of the beta-adrenergic blocker esmolol on myocardial dysfunction in septic rats

Sat Oct 01 00:00:00 EDT 2005

Cardioprotective effects of exercise training on myofilament calcium activation in ovariectomized rats.

Journal of applied physiology (Bethesda, Md. : 1985)

Bupha-Intr T,Wattanapermpool J

PA1-049 was used in western blot to study whether the cardioprotective effects of exercise training affects calcium activation in cardiac myofilament in ovariectomized rats

Sat May 01 00:00:00 EDT 2004

Effects of monocular deprivation on the expression pattern of alpha-1 and beta-1 adrenergic receptors in the kitten visual cortex.

Neuroscience research

Nakadate K,Imamura K,Watanabe Y

PA1-049 was used in immunohistochemistry to study the regulation of adrenergic receptors by sensory afferents in kitten brains

Fri Jun 01 00:00:00 EDT 2001

Bioinformatics

Protein Aliases: adrenergic receptor, beta 1; adrenergic, beta-1-, receptor; beta 1-adrenergic receptor beta 1-AR; beta 1-AR; Beta-1 adrenergic receptor; Beta-1 adrenoceptor; Beta-1 adrenoreceptor; cardiac beta adrenergic receptor

Gene Aliases: Adrb-1; ADRB1; ADRB1R; B1AR; beta-AR; BETA1AR; RATB1AR; RHR

UniProt ID: (Human) P08588, (Mouse) P34971, (Rat) P18090

Entrez Gene ID: (Human) 153, (Mouse) 11554, (Rat) 24925

Molecular Function: G-protein coupled receptor