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Invitrogen
eNOS Polyclonal Antibody
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FIGURE: 1 / 7
eNOS Antibody (PA5-16887) in ICC/IF
Immunofluorescence analysis of eNOS was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with eNOS Rabbit Polyclonal Antibody (Product # PA5-16887) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at... View More
Product Details
PA5-16887
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide derived from C-ternimal of human eNOS
Conjugate
Form
Liquid
Concentration
0.081 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.6, with 1% BSA
Contains
0.1% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA5-16887 targets eNOS in immunohistochemistry (paraffin) applications and shows reactivity with Bovine, Canine, Human, mouse, Porcine, and Rat samples. Suggested positive control is capillary endothelium and regenerative endothelium.
The PA5-16887 immunogen is a synthetic peptide derived from C-ternimal of human eNOS.
Target Information
Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cNOS; Constitutive NOS; e-NOS; EC-NOS; endothelial nitric oxide synthase; Endothelial NOS; eNOS; nitric oxide synthase; Nitric oxide synthase 3; nitric oxide synthase 3 (endothelial cell); Nitric oxide synthase, endothelial; NOS type III; NOSIII; OTTHUMP00000213183; unnamed protein product
Gene Aliases: ECNOS; eNOS; NOS3
UniProt ID: (Human) P29474
Entrez Gene ID: (Human) 4846
actin monomer binding
nitric-oxide synthase activity
protein binding
calmodulin binding
FMN binding
superoxide-generating NADPH oxidase activity
oxidoreductase activity
heme binding
tetrahydrobiopterin binding
arginine binding
cadmium ion binding
metal ion binding
flavin adenine dinucleotide binding
NADP binding
scaffold protein binding
angiogenesis
ovulation from ovarian follicle
in utero embryonic development
blood vessel remodeling
regulation of sodium ion transport
regulation of the force of heart contraction by chemical signal
regulation of systemic arterial blood pressure by endothelin
aortic valve morphogenesis
pulmonary valve morphogenesis
endocardial cushion morphogenesis
arginine catabolic process
nitric oxide biosynthetic process
potassium ion transport
calcium ion transport
mitochondrion organization
nitric oxide mediated signal transduction
blood circulation
regulation of blood pressure
negative regulation of cell proliferation
response to heat
response to hormone
negative regulation of platelet activation
positive regulation of gene expression
negative regulation of muscle hyperplasia
smooth muscle hyperplasia
removal of superoxide radicals
lung development
regulation of neurological system process
lipopolysaccharide-mediated signaling pathway
response to lipopolysaccharide
response to fluid shear stress
vasodilation
negative regulation of potassium ion transport
positive regulation of blood vessel endothelial cell migration
endothelial cell migration
cell redox homeostasis
positive regulation of Notch signaling pathway
positive regulation of angiogenesis
negative regulation of blood pressure
tetrahydrobiopterin metabolic process
nitric oxide metabolic process
negative regulation of smooth muscle cell proliferation
homeostasis of number of cells within a tissue
establishment of localization in cell
negative regulation of calcium ion transport
ventricular septum morphogenesis
negative regulation of biomineral tissue development
regulation of blood vessel diameter
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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