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|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the C-terminal portion of mouse iNOS.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-21054 detects iNOS in human, mouse, and rat samples.
PA1-21054 has been successfully used in immunohistochemistry (FFPE). Boil tissue sections in 10mM citrate buffer, pH 6.0 for ten minutes followed by cooling at room temperature for 20 minutes. A possible positive control for PA1-21054 is lung.
The PA1-21054 immunogen is a synthetic peptide corresponding to the C-terminal portion of mouse iNOS.
NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Notric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti-tumor and anti-pathogenic activities. Inducible NOS (iNOS) or macrophage NOS (mNOS) is a calcium/calmodulin independent and is expressed in activated macrophages and stimulated glial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
hepatocyte NOS; Inducible Nitric Oxide Synthase; inducible NO synthase; inducible NOS; MAC-NOS; macrophage NOS; nitric oxide synthase 2, inducible; nitric oxide synthase 2, inducible, macrophage; nitric oxide synthase 2A (inducible, hepatocytes); nitric oxide synthase, inducible; nitric oxide synthase, macrophage; NOS Type II; NOS, type II; peptidyl-cysteine S-nitrosylase NOS2
HEP-NOS; i-NOS; INOS; Inosl; NOS; Nos-2; NOS-II; NOS2; NOS2A