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Efficient separation and detailed analysis of recombinant proteins with POROS Affinity HPLC Columns
Thermo Scientific POROS affinity HPLC columns offer an innovative solution for efficient separation and detailed analysis of recombinant proteins. These columns are designed with advanced CaptureSelect affinity ligands immobilized on a robust POROS bead matrix, facilitating superior performance in high-throughput and high-specificity applications. Ideal for use in biotechnology and pharmaceutical research and manufacturing, these columns offer several key benefits:
- High binding capacity: Helps perform robust analysis over a wide sample load of recombinant proteins
- High-specificity analysis: Aims to deliver precise and detailed characterization of recombinant proteins
- Rapid mass transfer: Enables faster processing times and higher throughput
- Exceptional mechanical stability: Supports high flow rates and robust performance in demanding applications
- Versatility: Suitable for a wide range of recombinant proteins, helping enhance its utility in various research and development processes
- Reusability: Designed for multiple cycles for cost-effective and reproducible quantitation over hundreds of chromatography cycles
Affinity chromatography
Affinity chromatography is a technique used to separate biomolecules based on specific interactions between the molecule of interest and a ligand. In this process, the sample containing the target biomolecule is injected into the chromatography system and passed through a column filled with a stationary phase that has ligands specific to the target biomolecule. The target biomolecule binds to the ligands, while other molecules pass through the column. To elute the target biomolecule, a solution that disrupts the binding interaction, such as a buffer with a different pH or ionic strength, is used. The eluted biomolecule is then detected and quantified.
Chromatography chemistries
Thermo Fisher Scientific offers chromatography chemistries designed to deliver robust precision and reproducibility for biotherapeutic analysis. POROS resins were developed for high-resolution separations, while CaptureSelect ligands offer targeted specificity to HPLC workflows for consistent and reliable molecular characterization.
Explore how POROS resins deliver speed, resolution, and reliability for your most demanding HPLC analyses.
Discover how CaptureSelect ligands bring unmatched specificity and consistency to your analytical workflows.
POROS columns for recombinant proteins
POROS CaptureSelect affinity columns for protein analysis are designed to provide a precise and efficient method for purifying and characterizing recombinant proteins. This technology employs specialized CaptureSelect ligands on a durable POROS bead matrix, contributing to high binding capacity, fast processing times, and comprehensive analytical results, ideal for high-throughput biotechnology and biopharmaceutical applications.
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For research and development use only in support of FDA-regulated end uses. Not for diagnostic use or direct administration to humans or animals.