Thermo Scientific™

POROS™ CaptureSelect™ HSA Affinity Column, 4.6 x 50 mm

Catalog number:  4469165

 Related applications: Antibody & Cell Culture Production | Chromatography | Protein Purification & Isolation

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Analytical groups supporting biologic drug development require rapid, sensitive and precise methods for protein quantitation from cell culture, fermentation harvests, or purification intermediates. It is critical for biopharmaceutical manufacturers to be able to monitor bioreactor product titers to determine when to harvest or abort a reactor cycle.

Poros™ CaptureSelect™ HSA HPLC columns incorporate the advantages of high-speed Poros chromatography resins with the unique selectivity of CaptureSelect™ ligands. The combination allows three minute quantitation of HSA fusion proteins.


• Broad utility—POROS™ A, G and CaptureSelect™affinity ligands address most biological drug classes
• Rapid—POROS™ resins offer fast quantitation or purification of immunoglobulins and fusion proteins
• Hands-off operation—HPLC method can be automated for streamlined operation
• Robust—deliver high-performance purification over hundreds of chromatography cycles

High Speed with High Specificity
The combination of CaptureSelect™ HSA affinity ligands when combined with POROS™ 20 µm resin provides a high flow rate, high performance chromatography solution for the rapid quantitation and small-scale purification of HSA Fusion proteins.

POROS™ high performance chromatography allows for the quantitation of product from clarified harvest or partially purified intermediates—typically in under three minutes. Moreover, only minimal sample preparation (0.2 µm filtration) is required, and the eluted product is highly purified and suitable for further analysis including mass spectrometry.

POROS™ affinity chromatography columns are suitable for use with HPLC systems, which allows the automation of chromatography methods, data collection, and data analysis. In addition, the immobilized affinity ligands enable one method and buffer system to be used across a class of molecules, streamlining analytical approaches.

Multiple Cycles
Proper sample preparation, adherence to column operating instructions, and the use of an optimized column cleaning regime results in strong re-use performance. POROS™ Affinity Chromatography columns can be cycled hundreds of times for cost-effective and reproducible quantitation or purification.

HSA Column Specifications:
Protein Binding Capacity 2.6 mg/mL
Species Specifiity Human
Column Material PEEK™ polymer or stainless steel
Frit 2 µm, titanium
POROS™ Resin Cross-linked poly (styrenedivinylbenzene)
CaptureSelect™ Ligands Single-domain monospecific antibody fragment produced in Saccharomyces cerevisiae

For Research Use Only. Not for use in diagnostics procedures.


Binding Strength: 2.6 mg/ml dynamic binding capacity
Column Type: Affinity Column, PrePacked Column
Dimensions: 4.6 mm (diameter) x 50 mm (length)
Elution Volume - max: 1.6 ml
Elution Volume - min: 800 µl
Flow Rate: Up to 5000 cm⁄hr
For Use With (Equipment): HPLC
Format: Column
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Immobilized Phase: CaptureSelect™ HSA
Maximum Operating Pressure: 180 bar
Particle Size: 20 micron
Pore Size: 0.16 micron
Product Line: CaptureSelect™, POROS®
Product Size: 1 column
Reactivity: Human
Sample Type (Specific): Cell Culture Supernatants
Shipping Condition: Wet Ice
Support Matrix: Polystyrenedivinylbenzene
Target Molecule: HSA, HSA Fusion Proteins
Volume (Metric): 800 µl column volume

Contents & storage

Column includes EZ grip fittings and column test certificate

Store at 4°C DO NOT FREEZE


Manuals & protocols