POROS™ CaptureSelect™ FSH Affinity HPLC Columns

Thermo Scientific™

POROS™ CaptureSelect™ FSH Affinity HPLC Columns

Name: POROS™ CaptureSelect™ FSH Affinity HPLC Columns
SKU: 4481824

Analytical teams that support biologic drug development require rapid, sensitive, and precise methods for protein quantitation from cell culture, fermentations broths, or purification intermediates.

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製品番号（カタログ番号）	寸法
4481824	4.6 x 50 mm
4481822	2.1 x 30 mm
4481826	4.6 x 100 mm

3 オプション

製品番号（カタログ番号） 4481824

価格（JPY）

311,600

Each

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寸法:

4.6 x 50 mm

Analytical teams that support biologic drug development require rapid, sensitive, and precise methods for protein quantitation from cell culture, fermentations broths, or purification intermediates. It is critical for biopharmaceutical manufacturers to be able to monitor bioreactor product titers to determine when to harvest or abort a reactor cycle. POROS CaptureSelect FSH HPLC columns incorporate the advantages of high-speed POROS chromatography resins with the unique selectivity of CaptureSelect ligands, enabling 3-minute quantitation of human follicle stimulating hormone (FSH).

Features of this class of chromotography columns:

Broad utility—POROS A, G, and CaptureSelect affinity ligands address most biological drug classes
Rapid—POROS resins offer fast quantitation or purification of immunoglobulins and fusion proteins
Hands-off operation—HPLC method can be automated for streamlined operation
Robust—delivers high-performance purification over hundreds of chromatography cycles

High Speed with High Specificity

The combination of CaptureSelect FSH affinity ligand with POROS 20 μm resin provides a high flow-rate, high performance chromatography solution for the rapid quantitation and small-scale purification of FSH.

POROS high performance chromatography allows for the quantitation of product from clarified harvest or partially purified intermediates—typically in less than 3 minutes. Moreover, only minimal sample preparation (0.2 μm filtration) is required, and the eluted product is highly purified and suitable for further analysis, including mass spectrometry.

Automation

POROS affinity chromatography columns are suitable for use with HPLC systems, allowing for the automation of chromatography, data collection, and data analysis. In addition, the immobilized affinity ligands enable one method and buffer system to be used across a class of molecules, streamlining analytical approaches.

Multiple Cycles

Proper sample preparation, adherence to column operating instructions, and the use of an optimized column cleaning regime results in strong re-use performance. POROS affinity chromatography columns can be cycled hundreds of times for cost-effective and reproducible quantitation or purification.

For Research Use Only. Not for use in diagnostic procedures.

仕様

結合能1.5 mg/mL resin

カラムタイプAffinity

寸法4.6 x 50 mm

最大溶出量<800 μL

使用対象（アプリケーション）FPLC

高スループット適合性Automated Protocols

最大圧力180 bar

数量1 Column

サンプルタイプBiopharmaceutical Purification Sample, Bulk Drug Substance Sample, Cell Cultures

固定相Follicle Stimulating Hormone affinity ligand, humanspecific

標的分子Follicle Stimulating Hormone

容量（メートル法）0.8 mL

長さ（メートル法）50 mm

材料PEEK

粒子径20 μm

孔径2000 Å

製品ラインCaptureSelect, POROS

精製標的Follicle Stimulating Hormone

タイプColumn

Unit SizeEach

組成および保存条件

Column includes EZ grip fittings and column test certificate

Store at 4 °C. DO NOT FREEZE.

よくあるご質問（FAQ）

Are POROS analytical columns compatible with HPLC systems from Waters or Agilent?

All of the pre-packed POROS columns, regardless of column size or resin, have the same 10-32 female fittings on the column. Columns are compatible with Waters and Agilent HPLC systems. You will need 1/16 inch male fittings for the HPLC to attach the column.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

For any POROS analytical column, is the column compatible with an AKTA or Avant purification system?

All of the pre-packed columns, regardless column size or resin, have the same 10-32 female fittings on the column. They are all compatible with the AKTA instrument platform, however due to the column volume of the 2.1 mmD x 30 mmL columns (0.1 mL), these will not perform well in AKTA or Avant purification systems due to the relative size of the hold- up volume in these systems. The smallest column (PEEK or SS) that could feasibly be used in these systems is the 4.6mmD x 50mmL (0.8 mL) format.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Are the pre-packed POROS CaptureSelect or any POROS analytical columns compatible with an UPLC or is the use of these columns limited to HPLC?

UPLC is typically described as chromatography with sub-2 µm particles, which drives higher efficiency/better separation independent of operating flow rate. As particle size decreases, backpressure increases so the UPLC systems tend to have higher pressure ratings compared to HPLC systems. The flow rates on UPLC systems would be sufficient for POROS columns. Pump capabilities are typically 0.1-1.0 mL/min, so not as fast as a HPLC, but sufficient. Hold-up volumes on UPLC systems are lower than HPLCs, so this can help with sensitivity and efficiency. So from a pressure and flow standpoint there should be no challenge to operate a POROS CaptureSelect column on a UPLC system.

That being said, care should be taken running POROS columns on UPLC systems. Higher pressure safety interlock MUST be set at the rated column pressure, typically 180 bar. HPLC column hardware is not rated to operate at the static pressures possible and typical on UPLC systems. A clog or the like on a UPLC system could generate unsafe pressures on any HPLC-rated column before the default UPLC safety pressure interlock is tripped.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the physical or mechanical stability of POROS resins?

The physical stability of POROS resins is high. Customers have agitated, with overhead mixing and or bottom gassing for long periods of time (5-10 hours) and have performed hundreds of diaphragm pump passes during stall packs. Keep in mind that agitation with any device that creates grinding and sheer, such as with a magnetic stir bar, is not recommended for any type of resin.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What should I do if a POROS column dries out or air is pumped onto the column?

POROS resins rehydrate quite well. Here is the procedure that we recommend:

1. Flow 5% ethanol over the column at ?350 cm/hour for 3 CVs, followed by water or 0.1 M sodium chloride at 300 cm/hour for 3 CVs.
2. If baseline noise is still present, flow 20% ethanol over the column at 300 cm/hour for 3 CVs, followed by water or 0.1 M sodium chloride at 300 cm/hour for 3 CVs.
3. If you have established column qualification tests and specifications, perform the tests after rewetting.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

View all POROS™ CaptureSelect™ FSH Affinity HPLC Columns FAQs