POROS® CaptureSelect® FSH Affinity Column, 2.1 x 30 mm

Catalog number: 4481822

Applied Biosystems™  Related applications: Chromatography | Protein Purification & Isolation

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Description

Analytical teams that support biologic drug development require rapid, sensitive, and precise methods for protein quantitation from cell culture, fermentations broths, or purification intermediates. It is critical for biopharmaceutical manufacturers to be able to monitor bioreactor product titers to determine when to harvest or abort a reactor cycle. POROS® CaptureSelect® FSH HPLC columns incorporate the advantages of high-speed POROS® chromatography resins with the unique selectivity of CaptureSelect® ligands, enabling 3-minute quantitation of human follicle stimulating hormone (FSH).

Features of this class of chromotography columns:

• Broad utility—POROS® A, G, and CaptureSelect® affinity ligands address most biological drug classes
• Rapid—POROS® resins offer fast quantitation or purification of immunoglobulins and fusion proteins
• Hands-off operation—HPLC method can be automated for streamlined operation
• Robust—delivers high-performance purification over hundreds of chromatography cycles

High Speed with High Specificity
The combination of CaptureSelect® FSH affinity ligand with POROS® 20 µm resin provides a high flow-rate, high performance chromatography solution for the rapid quantitation and small-scale purification of FSH.

POROS® perfusion chromatography allows for the quantitation of product from clarified harvest or partially purified intermediates—typically in less than 3 minutes. Moreover, only minimal sample preparation (0.2 µm filtration) is required, and the eluted product is highly purified and suitable for further analysis, including mass spectrometry.

Automation
POROS® affinity chromatography columns are suitable for use with HPLC systems, allowing for the automation of chromatography, data collection, and data analysis. In addition, the immobilized affinity ligands enable one method and buffer system to be used across a class of molecules, streamlining analytical approaches.

Multiple Cycles
Proper sample preparation, adherence to column operating instructions, and the use of an optimized column cleaning regime results in strong re-use performance. POROS® affinity chromatography columns can be cycled hundreds of times for cost-effective and reproducible quantitation or purification.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Binding Strength: 1.5mg⁄ml resin
Column Type: Affinity Column
Elution Volume - max: < 100 µl
For Use With (Equipment): FPLC
Format: Individual
High Throughput Compatibility: Automated Protocols
Immobilized Phase: CaptureSelect™ FSH
Maximum Operating Pressure: 180 bar
Particle Size: 20 micron
Product Size: 1 column
Sample Type (Specific): Biopharmaceutical Purification Sample, Bulk Drug Substance Sample, Cell Cultures
Target Molecule: Follicle Stimulating Hormone

Contents & storage

Column includes EZ grip fittings and column test certificate

Store at 4 °C DO NOT FREEZE

Documents

Manuals & protocols

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