Peptide analysis often involves complex samples matrices, such as biological fluids or tissue extracts, which may contain a wide range of peptides with varying concentrations. Analyzing and identifying specific peptides of interest within this complexity can be challenging.
Peptide mapping analysis plays an important role in the characterization of biotherapeutics, enabling the confirmation of primary sequences and the identification of post-translational modifications (PTMs), such as phosphorylation or glycosylation. These modifications can significantly impact peptide behavior and complicate analysis.
You will need a chromatography column with excellent chromatographic performance that can retain both hydrophobic and hydrophilic peptides, like our new Hypersil GOLD Peptide column.
Advantages of this peptide column include:
- Unique bonded phase chemistry – a proprietary C18 bonded phase with a high surface area coverage, which provides a balance between hydrophobic and hydrophilic interactions. This allows for efficient separation and retention of a wide range of peptides, regardless of hydrophobicity or hydrophilicity.
- High purity silica – the narrow particle size distribution offers unmatched performance in peptide analysis.
What specialized techniques and expertise are required to identify and characterize PTMs in peptide analysis?
Monitoring of PTMs requires careful consideration of several key factors, including reproducibility, sensitivity, precision, and throughput. Hypersil GOLD Peptide columns provide outstanding performance and exceptional durability, making them the ideal choice for your peptide monitoring needs.
Hypersil GOLD Peptide column performance is assured through:
- Dual quality confirmation, including a peptide-based Quality Control (QC) test to deliver confidence in ultrapure silica, ligand bonding, and column packing for unparalleled sequence coverage and higher peak capacity compared to other peptide columns.
- Consistent and reliable results – chromatographically tested during QC in the manufacturing process to ensure column-to-column and lot-to-lot reproducibility.
How can you achieve greater sensitivity and lower limits of quantitation (LOQs) in peptide analysis with the Hypersil GOLD Peptide columns?
- 1.9 µm particle size produces sharp symmetric peak shape and peak area with higher efficiency, greater sensitivity and LOQs.
- Optimized peptide retention- strong retention time reproducibility, retained, and separated hydrophobic and hydrophilic peptides.
- Precise chromatographic resolution values for deamidated species in the PENNYK region.
You can read more about the benefits of using the Hypersil GOLD Peptide column for your peptide analysis in this application note and this flyer.
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