Calculations are crucial to help optimize productivity and support high-quality results in bioprocessing downstream processes. Downstream processing involves a series of purification steps, such as filtration, chromatography, and centrifugation, to isolate and purify the desired product from the raw bioreactor harvest. Calculations in these operations typically involve determining process yields, purification efficiencies, and the necessary parameters for each purification step. However, these calculations can often be complex and time-consuming, requiring significant manual effort from scientists, engineers, and other technical team members.
An addition to the Thermo Fisher Scientific website—the Bioprocessing Downstream Calculators—offers a streamlined and simplified approach to such complex calculations to help guide your downstream purification and buffer strategy.
Get to know the four calculators
The Bioprocessing Downstream Calculators are a series of four web-based tools specifically designed to generate biopharma downstream calculations quickly and efficiently for column dimension, process modeling, buffers, and column efficiency. This four-in-one tool brings together calculations needed for chromatography columns, resins, chemicals, process liquids, and buffers. The calculators leverage a collection of advanced algorithms and mathematical models built by Thermo Fisher Scientific technical specialists and practitioners to automate complex downstream calculations.
What’s the best part? As you make calculations, you can save your inputs and results by downloading them as a PDF for easy future reference.
Learn more about how this calculator works
Column dimension calculator
Calculate column dimensions by providing column diameter, length and/or volume parameters. Determine volumetric flow rate, flow velocity, and residence time using the flow parameter.
Process modeling calculator
Simulate the full chromatography process using this calculator. For each step, a buffer or solution is suggested as well as a few column volumes (CVs).
Buffer calculator
This buffer calculator provides formulation calculations based on the Henderson-Hasselbach equation to help make quick references and decisions for the process liquid and buffer solutions critical to the downstream bioprocessing operations
Column efficiency calculator
This calculator enables the calculation of column symmetry, plate count, reduced plate height, and HETP.
Why use the Bioprocessing Downstream Calculators?
- Time-saving efficiency: The calculators automate complex downstream calculations to help reduce the time and effort required. With its pre-programmed formulas, users can quickly input relevant data and obtain accurate results in a fraction of the time compared to manual calculations.
- Error reduction: By reducing the need for manual data entry and calculations, the Bioprocessing Downstream Calculators can help minimize the risk of mathematical errors. This can lead to increased accuracy to output reliable results and help reduce the likelihood of costly mistakes in downstream processes.
- Enhanced productivity: With streamlined calculations, bioprocessing professionals can allocate more time to other critical tasks, such as process optimization and troubleshooting.
- Scalability: The calculators support relevant downstream operational calculations for professionals working in volumes that are small, large, or both. This calculator can help optimize purification processes, from discovery to commercialization.
Check out how the Bioprocessing Downstream Calculators can help make your calculations fast and easy.
Related resources
- Explore chromatography and purification solutions
- Discover CGMP chemicals and service capabilities
- Learn about Gibco Process Liquids and Buffers
- See our entire portfolio of bioprocessing solutions
The data presented in the Downstream Bioprocessing Calculators is for informational purposes only, and based on user inputs. We do not endorse or warrant these results as accurate, comprehensive, or complete.
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