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Microbial Identification Kits and Libraries

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Root cause identification on demand

Engineered to facilitate accurate genotypic microbial identification in less than five hours, MicroSEQ PCR and Sequencing Reagent Kits help provide a streamlined, high-resolution workflow from DNA amplification through species-level identification. Designed for environmental monitoring and regulated quality control laboratories, these microbial identification kits are built to facilitate answers without unnecessary uncertainty, giving teams the clarity and confidence on demand they need when it matters most.

Each kit includes proprietary master mixes, optimized primers, and all required reagents to simplify implementation and support consistent PCR and sequencing performance. Validated for use with the SeqStudio family of genetic analyzers, MicroSEQ kits enable high-resolution Sanger sequencing across a broad range of bacterial and fungal organisms. Engineered to limit ambiguity, reducing repeat testing, and supporting root cause identification on demand, the MicroSEQ system helps laboratories improve predictability, scale operations efficiently, and reduce operational risk over time. The result is reliable, reproducible species-level identification that strengthens contamination investigations and supports confident regulatory decision-making.

 

MicroSEQ kits include:

PCR Kits

Sequencing Kits

2X Master Mix

Positive control DNA

Negative control DNA

3X Forward sequencing mix

3X Reverse sequencing mix

 

 

 

 

 

Bacterial identification: High-resolution 16S rRNA sequencing

For bacterial identification, the MicroSEQ system targets the 16S ribosomal RNA gene, a universally conserved genetic marker present in all bacteria. This well-established target combines conserved regions for precise PCR amplification with hypervariable regions that help provide strong discriminatory power during sequencing. The result is reliable, species-level bacterial identification across a broad range of organisms commonly encountered in environmental monitoring and quality control laboratories.

 

The MicroSEQ system helps provide flexibility to sequence either the first 500 base pairs of the 16S rDNA gene or the full 1,500 base pairs, depending on the level of resolution required. The 500 base pair option supports efficient, routine microbial identification workflows with predictable performance. When greater discrimination is needed for closely related organisms or in-depth contamination investigations, full-gene sequencing helps provide enhanced resolution and deeper phylogenetic insight. Validated reagent kits and curated reference libraries support both approaches, helping laboratories deliver answers without unnecessary uncertainty, strengthen root cause identification on demand, and reduce operational risk over time.

 

Figure 1. Hypervariable regions within the 16S rRNA gene. There are 9 hypervariable regions within the bacterial 16S gene, indicated in green. The conserved regions are indicated in grey.

Bacterial ID kits and libraries

Description

PCR kit

Sequencing kit

Library

# of entries

16sR full gene

MicroSEQ Full Gene 16S rDNA PCR Kit

MicroSEQ Full Gene 16S rDNA Sequencing Kit

MicroSEQ ID 16S rDNA Full Gene Library v2.1

1,262

16sR, first 500 base pairs

Fast MicroSEQ 500 16S rDNA PCR Kit

MicroSEQ 500 16S rDNA Sequencing Kit

MicroSEQ ID 16S rDNA 500 Microbial Library, v2022

2,100

MicroSEQ ID 16S rDNA 500 Supplemental Library, v2022

7,645

Fungal and yeast identification: LSU-D2 and ITS for enhanced species resolution

For fungal identification, the MicroSEQ system uses comparative sequence analysis of the LSU-D2 region and the Internal Transcribed Spacer regions to achieve accurate species-level classification. These ribosomal targets help provide the resolution required to differentiate among fungal organisms in environmental monitoring and regulated quality control settings. The ITS region offers greater variability than LSU-D2, generating more distinct sequence types and enabling higher discriminatory power for closely related fungal species.

 

Because fungal genomes may contain multiple copies of ribosomal DNA clusters, distinguishing meaningful phylogenetic differences from natural sequence variability is essential for dependable identification. The MicroSEQ fungal identification database is derived from carefully curated LSU-D2 rDNA sequences, supporting consistent and reproducible results in routine testing. By combining validated sequence targets with curated reference libraries, the MicroSEQ system is designed to help deliver answers without unnecessary uncertainty, enhance confidence in compliance, and help provide predictable fungal identification performance across laboratories and sites.

Fungal and yeast ID kits and libraries

Description

PCR kit

Sequencing kit

Library

# of entries

LSU-D2 rDNA

Fast MicroSEQ D2 LSU rDNA Fungal PCR Kit

MicroSEQ D2 rDNA Fungal Sequencing Kit

MicroSEQ ID Fungal Gene Library, v2022

1,737

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Explore the Analytics Knowledge Hub and discover a robust collection of webinars, articles, eBooks, infographics, and other digital resources to help you optimize analytical testing in your bioprocess workflow.
 

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