SYTOX® Dead Cell Stains Protocol
Membrane integrity—based cell viability assay
The SYTOX® Dead Cell Stains are nucleic acid stains for assessing cell viability with flow cytometry. Dead cells have bright fluorescence and live cells have dim fluorescence. SYTOX® Dead Cell Stains are available in five different colors.
This protocol can be used for:
- Identifying live and dead cells using a flow cytometer
This protocol should not be used for:
- Fluorescence microscopy
|1. Thaw vial of dye
|2. Add 1 mL cells to a flow cytometer tube
|3. Add 1µL dye to cells and mix well
|4. Incubate for 15 minutes
|5. Run cells on a flow cytometer
- Cell concentration should be 5 x 107 cells per mL
- No washing is required after staining
- Not compatible with fixation
- Dye stock solutions should be stored frozen
|Excitation/Emission (in nm)||444/480||488/525||547/570||546/647||640/658|
|Flow cytometer channel||DAPI||FITC||PE||PerCP||Alexa Fluor® 647|
|The SYTOX® Dead Cell Stain Sampler Kit (Cat. No. S34862) contains trial-sized samples of 5 different stains (50 tests per stain) to help researchers identify the best stain for their multicolor experiments.|
A mixture of heat-killed and live Jurkat cells were labeled with the SYTOX® Dead Cell stains. All stains were labeled according to the listed protocol. Samples were analyzed on a flow cytometer equipped with a 488 nm laser, a 405 nm laser, or a 633 nm laser, and fluorescence emissions were collected using the appropriate filters.
For Research Use Only. Not for use in diagnostic procedures.