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Invitrogen
Cyclic AMP Competitive ELISA Kit
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Product Specifications
Analytical sensitivity
0.037 pmol/mL
Assay range
0.078-20 pmol/mL
Sample type/volume
Cell Lysate
100 µL
Tissue Homogenate
100 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr
Homogenous (no wash)
No
Interassay CV
7.1%
Intraassay CV
6.5%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96-well plate
Detection Antibody
AP Conjugate
Neutralizing Reagent
Triethylamine
Acetic Anhydride
Standard
20X Wash Buffer
pNpp Substrate Solution
Stop Solution
Plate Sealer
Detection Antibody
AP Conjugate
Neutralizing Reagent
Triethylamine
Acetic Anhydride
Standard
20X Wash Buffer
pNpp Substrate Solution
Stop Solution
Plate Sealer
Shipping conditions
Dry ice
Storage
-20°C
Protein name
cAMP
Target site-specificity
Protein family
Other Proteins
Assay kit format
Competitive ELISA
Label or dye
AP
About This Kit
The cyclic AMP Competitive ELISA quantitates cAMP in cell or tissue lysates. The assay will exclusively recognize both natural and recombinant cyclic AMP.
Principle of the method
The cyclic AMP solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is inversely proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Cyclic adenosine monophosphate (cAMP) plays a key role as an intracellular second messenger for transduction events that follow a number of extracellular signals. The G-Protein Coupled Receptors (GPCR) is the largest family of cell surface receptors. They can be activated by different ligands, such as neurotransmitters, hormones, ions, small molecules, peptides, and other physiological signaling molecules. Typically, the binding of the ligands to its receptor resulting in the activation of G-proteins, in return, activates the effector adenylyl cyclase evoking the production of cAMP. The activation of a protein kinase by cAMP results in the phosphorylation of substrate proteins. Currently successful drugs in marketing have been developed to target these receptors. Among the GPCRs, ~367 receptors are potential drug development targets, but only about 20 have been used to generate therapeutically and commercially successful drugs so far. Because the involvement of cAMP can amplify the response of the ligand binding, the second messenger cAMP has been largely employed to monitor the activation of the GPCR to facilitate the therapeutic drug discovery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases : Cyclic AMP
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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