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Invitrogen
Equine TNF alpha Uncoated ELISA Kit
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Product Specifications
Analytical sensitivity
<15.6 pg/mL
Assay range
15.6-1,000 pg/mL
Sample type/volume
Supernatant
100 µL
Hands-on time
2 hr 50 min
Time-to-result
24 hr
Homogenous (no wash)
No
Instrument
Absorbance Microplate Reader
Product size
5 x 96 Tests
Contents
Coating Antibody
Lyophilized Recombinant Standard
Detection Antibody
Streptavidin-HRP
Substrate Solution
Ready to use Stop Solution
0.16M Sulfuric Acid
Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
Lyophilized Recombinant Standard
Detection Antibody
Streptavidin-HRP
Substrate Solution
Ready to use Stop Solution
0.16M Sulfuric Acid
Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
TNF alpha
Species (tested)
Horse
Assay kit format
Uncoated ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Equine TNF-alpha Uncoated ELISA Kit contains pre-matched antibody pairs and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of equine TNFalpha.
Principle of the method
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Target information
TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : TNF, TNF-a, TNF-alpha, TNFA, TNFSF2, TNLG1F
Gene ID : (Horse) 100033834
Gene symbol : TNF
Protein Aliases : Cachectin, DADB-70P7.1, TNF alpha, TNF superfamily, TNF α, TNFα, TNF-a, TNF-alpha, Tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor ligand 1F, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
UniProt ID (Horse) P29553
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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