Prepacked Chromatography Columns

On this page:

Selecting chromatography columns for biomolecule separation

Chromatography columns separate proteins, nucleic acids, and other biomolecules. Column selection depends on the stationary phase, operating pressure, and scale of use. Thermo Fisher Scientific supports accelerated speed to market, reproducibility and ease of use from development through manufacturing with prepacked columns.

Prepacked columns

Prepacked chromatography columns aim to simplify downstream workflows by reducing the need for manual packing. Available in multiple formats and volumes, these columns are packed with resins to facilitate purification across research and process development. Options include RoboColumn™, GoPure, and MiniChrom formats, offering flexibility for evaluation and scale-up.

Choose the right chromatography column for your workflow

Chromatographic column design influences recovery and scalability, so matching the format to the workflow is crucial when transitioning from development to manufacturing. The RoboColumn format is intended for automated high-throughput screening with robotic systems, while GoPure and MiniChrom formats are commonly used for resin evaluation and bench-scale development. Preparative prepacked columns are designed to extend these studies from bench-scale to pilot scale and beyond.
 

Column type Application Volume Diameter Bed height
GoPure small-scale prepacked column
  • Process development
  • Bench-scale screening
  • Preparative chromatography
  • 1 mL
  • 5 mL
  • 0.5 cm
  • 0.8 cm
  • 5 cm
  • 10 cm
MiniChrom column
  • Process development
  • Bench-scale screening
  • Preparative chromatography
  • 1 mL
  • 5 mL
  • 0.8 cm
  • 2 cm
  • 10 cm
Prepacked column
  • Preparative chromatography
  • 1 mL
  • 5 mL
  • 0.7 cm
  • 1.4 cm
  • 0.7 cm
  • 1.4 cm
RoboColumn prepacked column
  • High-throughput screening
  • Process development
  • 50 µL
  • 200 µL
  • 600 µL
  • 0.5 cm
  • 0.5 cm
  • 0.25 cm
  • 1 cm
  • 3 cm

GoPure small-scale prepacked columns

GoPure columns offer prepacked formats for resin screening and process development. Available in 1 mL and 5 mL sizes, they support process development with consistent bed heights for reliable scale-up.


MiniChrom columns

MiniChrom columns are prepacked formats designed to mimic process-scale columns. Offered in 1 mL and 5 mL sizes, they are used for method development and scale-down studies.


 

RoboColumn prepacked columns

RoboColumn formats are prepacked in 8-column strips for automated, parallel separations on robotic liquid handling systems. Available in 50 µL, 200 µL, and 600 µL volumes, these columns support high-throughput screening and process development.

Explore chromatography techniques

Chromatographic techniques—such as affinity, ion-exchange, hydrophobic interaction, and mixed-mode chromatography—are used at different stages of purification. Each mode applies distinct binding principles, making it important to match the technique with your target molecule and process needs.

Discover other chromatography products

Thermo Fisher Scientific offers additional chromatography products to optimize purification. Explore resins, membranes, and screening plates developed to complement column-based workflows and facilitate flexibility across scales.

CaptureSelect and POROS Technologies

CaptureSelect affinity resin leverages highly specific ligands for the capture of antibodies, proteins, viral vectors, and other biomolecules. POROS resin uses tuned chemistry on a rigid backbone with large throughpores for process-scale separations, offering high resolution and capacity.

Chromatography resins

Chromatography resins are available for affinity, ion exchange (IEX), hydrophobic interaction (HIC), and mixed-mode workflows. Formats span from evaluation tools to production-scale resins applied in cGMP biomanufacturing.

 

Membrane Chromatography

Membrane chromatography supports higher flow rates and shorter processing times compared to traditional packed-bed resin and can help accelerate biologics production from development through manufacturing.

96-Well Screening Plates

A 96-well plate format allows scientists to streamline resin evaluation by running multiple purifications in parallel. This enables early comparison of binding and wash conditions before moving to larger-scale studies.

Chromatography resources

Chromatography resources offer protocols, application notes, and videos that cover resin selection, column formats, and purification strategies. Content spans ion exchange, hydrophobic interaction, mixed-mode, and affinity chromatography, including Protein A chromatography.

RoboColumn™ is a registered trademark of Repligen Corporation.

For Research Use Only. Not for use in diagnostic procedures.