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3 | 488 | 660/20 | 492 | 665 | (in buffer) 5 | flow cytometry |
Invitrogen NovaFluor Blue 660 dyes are available with two different levels of brightness—NovaFluor Blue 660-40S and NovaFluor Blue 660-120S—that allow differential matching of the dye brightness to the antigen density or abundance without panel redesign. NovaFluor Blue 660-120S dye is the brighter dye. NovaFluor Blue 660-120S dye can be used to replace PE-tandem dyes, such as PE-Cy5 and PE/Fire™ 640, using the 488 nm blue laser line for excitation. Because PE-tandem dyes are cross-excited by both blue and yellow-green lasers, the replacement of these dyes with NovaFluor Blue 660-120S dye can allow detection of an additional marker labeled with, for example, NovaFluor Yellow 660 dye.
The macromolecule-based NovaFluor Blue 660-120S dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C. Use NovaFluor dyes with CellBlox Plus Blocking Buffer to reduce background and to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.
We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.
NovaFluor dyes are built using Phiton technology and are compatible with both spectral flow cytometry and traditional flow cytometry. NovaFluor dyes exhibit narrow emission spectra and minimal cross-laser excitation, which helps reduce spectral spillover for better marker resolution. In addition, their unique spectral signatures can provide the opportunity to detect additional markers in flow cytometry panels by opening up previously unusable channels.
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
A handy reference poster featuring the broad range of our available dyes and labeling reagents.
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
A tool for selecting the optimal fluorescent dyes for your Invitrogen EVOS cell imaging systems.
Cy™ is a trademark or registered trademark of GE Healthcare.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.
For Research Use Only. Not for use in diagnostic procedures.