Search

  • Contact Us
  • Quick Order

Sample Preparation and Automation Solutions for Contaminant and Impurity Testing

On this page:

Sample prep solutions for bioprocessing workflows

What is PrepSEQ chemistry?

 

Applied Biosystems PrepSEQ chemistry is a universal sample preparation workflow for contaminant and impurity testing designed to recover and purify nucleic acids from complex bioprocessing samples. Compatible with multiple applications, PrepSEQ chemistry supports workflows including:

  • Residual host cell DNA quantitation
  • Mycoplasma detection
  • Minute Mouse Virus (MMV) detection
  • Viral contaminant detection

Using magnetic bead-based chemistry, PrepSEQ kits efficiently isolates nucleic acids while helping remove proteins, salts, buffers, and other process-related contaminants that may interfere with downstream analysis. The purified DNA or RNA can then be used in qPCR and other molecular testing workflows.

Why choose PrepSEQ chemistry?

PrepSEQ chemistry is designed to help laboratories simplify sample preparation while improving workflow consistency across a wide range of contaminant and impurity testing applications.

 

Key features

  • Easy-to-use protocol featuring magnetic bead-based technology for efficient sample extraction, cleanup, and elution
  • Sensitive isolation of genomic DNA from the total lysate of test samples
  • Flexible automation options ranging from manual processing to low-, medium-, and high-throughput workflows
  • Accurate impurity quantitation of residual host cell DNA when paired with with the Applied Biosystems resDNASEQ Quantitative Residual Host Cell DNA Kit
  • Accurate determination of contaminant presence or absence when paired with the Applied Biosystems MycoSEQ Mycoplasma Detection Kit

How PrepSEQ Works

PrepSEQ chemistry uses magnetic bead-based purification to isolate nucleic acids from complex bioprocessing samples. The workflow follows six simple steps that can be performed manually or automated depending on your laboratory's throughput requirements.

Samples are lysed to release nucleic acids from cells, viral particles, or other biological material, creating a total lysate for purification.

Magnetic particles and a proprietary binding solution are added to the lysate, allowing DNA or RNA to selectively bind to the magnetic beads while unwanted sample components remain in solution.

The nucleic acid-bound magnetic particles provide an efficient mechanism for separating the target DNA or RNA from complex sample matrices while maintaining high recovery.

A series of purification washes removes proteins, salts, inhibitors, and other process-related contaminants, helping improve nucleic acid purity for downstream molecular analysis.

A magnetic field captures the nucleic acid-bound particles while contaminants are removed, simplifying sample cleanup and minimizing sample loss.

Purified nucleic acids are released from the magnetic particles into an optimized elution buffer, producing a PCR-compatible nucleic acid solution ready for downstream analysis.

The purified DNA or RNA is suitable for a variety of downstream contaminant and impurity testing applications, including:

  • Residual host cell DNA quantitation
  • Mycoplasma detection
  • Minute Mouse Virus (MMV) detection
  • Viral contaminant detection
  • Additional qPCR-based bioprocessing workflows

Choose the right PrepSEQ sample preparation kit

PrepSEQ sample preparation kits are designed to support a wide range of contaminant and impurity testing workflows. Whether you're preparing samples for residual host cell DNA quantitation, mycoplasma detection, viral contaminant testing, or nucleic acid recovery from complex bioprocessing samples, there is a PrepSEQ kit designed to fit your application.

 

Each kit uses the same proven magnetic bead-based chemistry while offering application-specific protocols optimized for different sample types, targets, and automation options.

 

Use the guide below to identify the PrepSEQ option that best aligns with your testing workflow.
 

PrepSEQ kit Best-fit use Typical sample prep need
PrepSEQ Residual DNA Sample Preparation  Residual host-cell DNA quantitation Recovery of residual DNA from complex samples containing proteins, salts, buffers, or other process-related components.
PrepSEQ Nucleic Acid Sample Preparation Kit  DNA and RNA recovery from diverse bioprocessing sample types Extraction of nucleic acids from cell culture, raw material, in-process, or downstream purification samples.
PrepSEQ 1-2-3 Nucleic Acid Extraction Kit  Mycoplasma and MMV sample preparation workflows Magnetic particle-based DNA extraction from mycoplasma cells or MMV-containing samples.
PrepSEQ Express Nucleic Acid Extraction Kit Cartridge-based contaminant testing workflows Automated extraction of DNA and/or RNA from mycoplasma cells or viral particles using prefilled cartridges.

 

Not sure which kit is right for your workflow?

Explore the complete PrepSEQ kit portfolio to compare applications, sample types, automation compatibility, and recommended workflows.

Manual or automated sample preparation?

PrepSEQ solutions are well suited for a wide range of applications in the biopharma industry. These applications include residual host cell DNA quantitation, residual plasmid DNA quantitation, mycoplasma detection, and viral detection. These comprehensive solutions enable you to address all your analytical needs with confidence and precision.

 

 Our technology helps ensure the recovery of all forms of nucleic acid, including double-stranded DNA, single-stranded DNA, and RNA. This complete nucleic acid recovery capability allows for accurate and efficient analysis, supporting a variety of testing and research applications.

Manual workflows may fit when:

  • Sample volume is limited
  • Throughput needs are low
  • The lab does not require walkaway automation
  • A specific protocol is already established manually

     

KingFisher systems may fit when:

  • The lab needs plate-based magnetic bead automation
  • Throughput requirements are higher
  • PrepSEQ workflows need to scale across more samples
  • The workflow benefits from 24- or 96-well processing

AutoMate Express may fit when:

  • The lab wants a closed, cartridge-based workflow
  • The workflow uses PrepSEQ Express chemistry
  • The testing need involves defined contaminant workflows
  • Simplicity and reduced hands-on time are priorities

For Research Use Only. Not for use in diagnostic procedures.

Analytics Knowledge Hub


Explore the Analytics Knowledge Hub and discover a robust collection of webinars, articles, eBooks, infographics, and other digital resources to help you optimize analytical testing in your bioprocess workflow.
 

Request a technical consultation

No matter your questions, we are here to help. Our experienced team of scientists and technical specialists is ready to assist you in choosing the right solution and products. Complete this form to start the conversation.
 

 

For Research Use Only. Not for use in diagnostic procedures.