PrepSEQ™ 1-2-3 Mycoplasma Nucleic Acid Extraction Kit

Catalog number: 4443789

Applied Biosystems™  Related applications: Contaminant & Impurity QC Testing | Diagnostic Testing | Mammalian Cell Culture

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Description

The Applied Biosystems® PrepSEQ™ 1-2-3 Nucleic Acid Extraction Kit rapidly extracts Mycoplasma genomic DNA from 100µl sample volumes of research cell lines, stem cell lines, and cell and tissue therapy samples. In the PrepSEQ™ 1-2-3 Kit protocol, a rapid procedure recovers DNA from total lysate of the test sample using magnetic-bead based technology. The Myco Scan Kit is optimized for use with the PrepSEQ 1-2-3 Kit.

- High recovery of Mycoplasma genomic DNA from mammalian cell culture samples
- Rapid easy to use protocol results in under 2 hours
- Compatible with the Myco Scan Mycoplasma Detection assay
- Protocol available for automated sample preparation

High DNA Recovery Sample Preparation
Optimized for use with the Myco Scan Kit, the PrepSEQ 1-2-3 Nucleic Acid Extraction Kit uses proprietary magnetic bead-based separation technology to extract Mycoplasma DNA from mammalian cell lines. The PrepSEQ 1-2-3 Kit extracts Mycoplasma genomic DNA from research cell lines, stem cell lines, cellular and tissue therapy samples with high efficiency.

The protocol is recommended for use with samples with a starting volume of 100 µl with a maximum cell density of 106 cells. The PrepSEQ 1-2-3 Kit provides an easy to use procedure consisting of 1) RNase treatment 2) Proteinase treatment and 3) DNA extraction using the magnetic bead technology (Figure 1). The kit isolates Mycoplasma genomic DNA with high sensitivity from total lysate of test sample. The PrepSEQ 1-2-3 Kit and the Myco Scan Kit allow routine screening of high-value cell lines using an easy, rapid and sensitive protocol. Figure 2 demonstrates how a sample that was spiked with low levels of Mycoplasma bovoculi DNA was found negative for Mycoplasma contamination using a commercially prepared kit. When using the PrepSEQ 1-2-3 Kit and the Myco Scan Kit, the correct positive call for Mycoplasma contamination was made.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Final Product: Genomic DNA
High Throughput Compatibility: High Throughput-Compatible
Isolation Technology: Magnetic Bead
Product Line: PrepSEQ™
Product Size: 100 reactions
Purification Time: 2 hrs
Sample Type (General): Liquid Samples (e.g. Serum)
Sample Type (Specific): Cell Culture Supernatants
Scale: Mini
Target Organism Class: Mycoplasma spp.

Contents & storage

Includes buffers, magnetic beads and enzymes for DNA extraction for Mycoplasma DNA extraction

Documents

Manuals & protocols