96-Well Screening Plates for Bioprocessing Purification

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High-throughput screening for optimizing downstream purification workflows

Developing purification conditions can be time-consuming when multiple variables must be tested for yield and purity. POROS GoPure 96-well screening plates allow for rapid, parallel evaluation of multiple samples or process conditions within a single experiment. This format supports high-throughput screening (HTS) and buffer optimization through consistent, reproducible comparative results. The same high-performance POROS and CaptureSelect resins used in the plates are also available in pre-packed columns and bulk formats for easy scale-up from screening to process development and production.

96-well screening plates for AAV purification

Adeno-associated virus (AAV) purification involves separating capsids from process-related impurities. The POROS GoPure 96-well screening plates enable an efficient platform for evaluating multiple wash and elution conditions while using less sample material than traditional column formats. This approach allows rapid assessment of parameters influencing AAV capsid recovery and purity.

96-well screening plates for antibody purification

POROS GoPure 96-well screening plates enable high-throughput, consistent purification of monoclonal antibody (mAb) candidates and systematic evaluation of purification parameters. Particularly valuable for bispecific antibodies (bsAb), they allow rapid screening of constructs and buffer conditions to maximize recovery of correctly assembled, functional molecules.

Learn how these and other tools can support process development at different stages.
 

96-well screening plates for mRNA purification

Messenger RNA (mRNA) purification reduces impurities generated during in vitro transcription (IVT) reactions. POROS GoPure 96-well screening plates are designed to evaluate the loading, wash, and elution conditions to help identify optimal purification parameters.

See how these and other tools integrate within the workflow.

Choose the resin screening plate for your molecule type

Choosing the correct plate for early screening and process design requires matching the resin to your target molecule. Options are available for AAV, antibody, and mRNA workflows.

 

Resin plate selection by application

Application

Plate type

Resin volume 

Intended use

Key attributes

AAV purification

POROS GoPure AAVX or AAV9 screening plates

AAVX: 20 µL

AAV9: 20 µL

Designed for viral vector purification and targeting AAV capsids

Rapid evaluation of parameters while conserving sample material

Antibody purification

POROS GoPure MabCaptureC or CaptureSelect GoPure FcXP screening plates

MabCaptureC: 20 µL or 50 µL

FcXP: 20 µL

Tailored for mAb purification

Helps accelerate mAb and bsAb development with high-throughput screening of molecules and buffers

mRNA purification

POROS GoPure Oligo(dT)25 screening plates

Oligo(dT)25: 20 µL

Used for mRNA capture through poly(A) tail hybridization

Evaluation of loading, wash, and elution parameters to enhance mRNA purification efficiency

Ordering information

Catalog # Name Size Price Qty
A65811 POROS™ GoPure™ Oligo (dT)25 Screening Plate, 96-well, 20 μL Each
1,072.00

Supporting diverse modalities and applications

ADCs

Antibody-Drug Conjugates (ADCs) workflows rely on chromatography steps to separate complex conjugates and reduce impurities. These approaches support consistent production of ADCs for therapeutic research and biomanufacturing.
 

Cell Therapy

Cell therapies utilize living cells as treatments, necessitating strict control over inputs and processing steps. Chromatography is applied to prepare ancillary materials, such as cytokines or viral vectors, that are used during cell manufacturing.
 

Gene Therapy

Gene therapy often relies on viral vectors, such as adeno-associated virus (AAV) or lentivirus, to facilitate the transfer of genetic material. Chromatography separates drug product from product- and process-related impurities in both capture and polishing steps.

mAbs

Monoclonal antibody purification typically starts with Protein A chromatography to capture the antibody from cell culture fluid. Additional steps, such as ion exchange, are used to help reduce aggregates, variants, or process-related impurities.
 

mRNA

Chromatography in mRNA production separates full-length transcripts from double-stranded RNA and truncated products. The purified RNA can then be used in formulation or downstream steps, such as lipid nanoparticle assembly.
 

Vaccines

Chromatography is often used in vaccine manufacturing for both the capture and polishing of the vaccine drug product. The choice of method depends on the platform, for example, protein subunits, viral vectors, or nucleic acid vaccines.
 

Explore more chromatography techniques

Chromatography is a method in bioprocessing used to separate biomolecules based on differences in physical and chemical properties. Thermo Fisher Scientific offers a range of chromatography resin modalities, including affinity, ion exchange (IEX), hydrophobic interaction (HIC), and mixed-mode. Each can be applied during capture, intermediate, or polishing steps to enhance resolution or yield at specific stages of purification.
 

Discover other chromatography products

Thermo Fisher Scientific offers chromatography products that support purification across different process stages and molecule types. Resins include affinity, IEX, HIC, mixed-mode formats. The portfolio features POROS and CaptureSelect resins, pre-packed chromatography columns, and membrane devices that collectively support purification of antibodies, viral vectors, mRNA, and recombinant proteins.

CaptureSelect and POROS Technologies

CaptureSelect and POROS resins support the purification of biologics, including antibodies, viral vectors, and recombinant proteins. CaptureSelect resins offer selectivity and gentle elution, while POROS resins offer speed and scalability for consistent large-scale chromatography performance.

Chromatography Columns

Pre-packed columns simplify setup by reducing the need for on-site column packing. Formats range from bench-scale to process-scale and include resins for capture, intermediate, and polishing steps in purification workflows.


 

Chromatography Resins

Chromatography resins are available for affinity, ion exchange (IEX), hydrophobic interaction chromatography (HIC), and mixed-mode applications. Formats range from early-stage evaluation tools to production-scale resins used in CGMP biomanufacturing.

Membrane Chromatography

Membrane chromatography offers higher flow rates and shorter processing times compared to traditional packed-bed resins and can help accelerate biologics production from development to manufacturing.

 

Frequently asked questions

A 96-well screening plate is a high-throughput (HTP) tool that serves two potential functions. It allows for the purification of multiple samples in parallel, to help accelerate candidate screening. It can also be used to speed up process development by permitting simultaneous evaluation of various buffer conditions before moving to on-column chromatography experiments.

The maximum well volume is 800 µL. The recommended working volume per well should be between 100 and 400 µL based on the purification step. The resin volume is consistent in each well of the plate; resin volumes vary between 20 and 50 µL depending on the resin or plate type.

The frit is made of a 25 µm polyethylene filter. The screening and collection plates are made from polypropylene.

Thermo Scientific Adhesive PCR plate seals are recommended for 96-well screening plates.

POROS GoPure 96-well screening plates meet ANSI/SLAS Standards 1–4 (2004) for microplate design. They are compatible with Thermo Scientific Nunc 96-Well Polypropylene DeepWell™ Sample Processing and Storage Plates with Shared-Wall Technology. The dimensions are consistent across systems, and the plates align easily with automated liquid handlers, offering dependable sample collection during resin screening.

The plates are compatible with centrifugation, liquid handling systems, and vacuum manifolds. See our user guide for conditions and equipment recommendations.

A standard 96-well plate is a general laboratory tool used for sample handling, storage, or assays. A 96-well screening plate is pre-packed with chromatography resin for process development. Each well functions as an individual batch binding experiment to investigate the loading, wash, and elution conditions used to define purification parameters.

Not sure which resin is right for you?

The Bioprocess Resin Selection Guide is an interactive guide that helps scientists evaluate chromatography resins based on molecule type, application, and scale. Identify products that meet your purification goals and process needs by filtering data on resin chemistry, capacity, and operating conditions.
 

Chromatography resources

Access application notes, protocols, and videos that support resin evaluation and process development. Topics include resin selection, column operation, method transfer, and troubleshooting for antibodies, viral vectors, and mRNA. These materials offer guidance for downstream workflows, spanning from early research to manufacturing.
 

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.