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MHC Class II (I-A) Monoclonal Antibody (NIMR-4), PE, eBioscience™
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FIGURE: 1 / 7
MHC Class II (I-A) Antibody (12-5322-81) in Flow
Nature communications 2018 -
Fig. 9 Physical interaction between TMPs and the DC surface is critical for TMP-mediated DC activation. a Schematic diagram of the transwell system (left). After co-incubation for 2 h, DCs were acid washed and evaluated for TCRbeta and V5G transfer (upper panel), followed continued incubation for 24 h. The expression of MHC class II, CD80, CD86, and CD40 on DCs (CD11c + ) was analysed by flow cytometry an... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
12-5322-81
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2b
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
NIMR-4
Conjugate
PE
PE
PE
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.1 mg/mL
Amount
50 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The monoclonal antibody NIMR-4 reacts specifically with a non-polymorphic I-A-encoded epitope on MHC Class II antigens. Class II antigens are predominantly expressed on antigen-presenting cells including B lymphocytes, macrophages, dendritic cells and certain epithelial cells. MHC Class II molecules are important for the selection of the TCR repertoire during the processes of positive and negative selection. MHC Class II expression is required for the positive selection of CD4+ T cells.
Applications Reported: This NIMR-4 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This NIMR-4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
MHC class II molecules are heterodimers of non-covalently associated alpha (31-34 kDa) and beta (26-29 kDa) chains. Major histocompatibility complex class II antigen presentation requires the participation of lysosomal proteases in two convergent processes. First, the antigens endocytosed by the antigen presenting cells must be broken down into antigenic peptides. Second, class II molecules are synthesized with their peptide-binding site blocked by invariant chain (Ii), and they aquire the capacity to bind antigens only after Ii has been degraded in the compartments where peptides reside. MHC class II molecules present exogenously derived antigen to CD4+ T lymphocytes, which are usually T helper cells. CD4 interacts with non-polymorphic residues of MHC class II.
HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: class II Ia antigen A-beta; H-2 class II histocompatibility antigen, A beta chain; H2-IA-beta cell surface glycoprotein; histocompatibility 2, class II antigen A, beta, promoter 1; I-Ar MHC class II molecule beta chain; major histocompatibility complex class II beta chain; MHC class II H2-IA-beta-psi; MHC H2-I-A-beta chain; MHC NOD beta chain precursor; MHC-II I-A(d) beta; response to metastatic cancers 1; unnamed protein product
Gene Aliases: Abeta; H-2Ab; H2-Ab; I-Abeta; Ia-2; Ia2; IAb; Rmcs1
UniProt ID: (Mouse) P14483
Entrez Gene ID: (Mouse) 14961
immune system process
peptide antigen assembly with MHC class II protein complex
immune response
antigen processing and presentation
antigen processing and presentation of exogenous peptide antigen via MHC class II
antigen processing and presentation of peptide antigen
positive regulation of immune response
positive regulation of T cell activation
Disclaimer
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