Thermo Scientific™ Pierce™ Streptavidin, Horseradish Peroxidase Conjugate includes streptavidin in a purified form conjugated to peroxidase for substrate-based detection.
Streptavidin, Horseradish Peroxidase Conjugate enables detection of biotinylated antibodies and other probes in a variety of standard assay methods, including western blotting, ELISA, immunohistochemistry, and fluorescence imaging. Streptavidin, Horseradish Peroxidase conjugate is supplied as lyophilized powder in phosphate-based buffer for immediate reconstitution with water.
Streptavidin is a biotin-binding protein originally isolated from Streptomyces avidinii. In contrast to avidin, streptavidin has no carbohydrate and has a mildly acidic pI of 5. Pierce streptavidin products use a recombinant form of streptavidin having a mass of 53,000 daltons and a near-neutral pI. Streptavidin is a tetrameric protein, with each subunit binding one molecule of biotin with affinity similar to that of avidin. Guanidinium chloride will dissociate avidin and streptavidin into subunits, but streptavidin is more resistant to dissociation.
Recommended for use in ELISA (0.1 µg/mL), immunohistochemistry (1-10 µg/mL), and western blot (2 µg/mL) applications.