Streptavidin Protein

Catalog number: 21122

Thermo Scientific™  Related applications: Cellular Imaging | Protein Assays and Analysis

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Description

Thermo Scientific™ Pierce™ Streptavidin is a purified unconjugated recombinant protein.

Features include:
Recombinant streptavidin—high-quality protein, 53 kDa, pI = 6.8–7.5
Pure protein—choose pure, unconjugated protein for biotin blocking and controls

Purified recombinant streptavidin enables detection of biotinylated antibodies and other probes in a variety of standard assay methods, including western blotting, ELISA, immunohistochemistry, and fluorescence imaging. Purified streptavidin is supplied lyophilized in phosphate-based buffer for immediate reconstitution with water.

Streptavidin is a biotin-binding protein originally isolated from Streptomyces avidinii. In contrast to avidin, streptavidin has no carbohydrate and has a mildly acidic pI of 5. Pierce™ streptavidin products use a recombinant form of streptavidin having a mass of 53,000 daltons and a near-neutral pI. Streptavidin is a tetrameric protein, with each subunit binding one molecule of biotin with affinity similar to that of avidin. Guanidinium chloride will dissociate avidin and streptavidin into subunits, but streptavidin is more resistant to dissociation.

Applications: ELISA (assay dependent), western blot (assay dependent)

Related products
Pierce™ Streptavidin, Horseradish Peroxidase Conjugated
Pierce™ Streptavidin, Alkaline Phosphatase Conjugated
Pierce™ Streptavidin, Maleimide-Activated
Pierce™ Streptavidin, Hydrazide-Activated

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Label or Dye: Unlabeled
Product Size: 1 mg
Target Molecule: Biotin

Contents & storage

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2 to 8°C.

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Manuals & protocols

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