Streptavidin Protein, AP

Catalog number: 21324

Thermo Scientific™  Related applications: Cellular Imaging | Protein Assays and Analysis

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Description

Thermo Scientific™ Streptavidin, Alkaline Phosphatase Conjugate includes streptavidin in a purified form, conjugated to alkaline phosphatase for substrate based detection.

Streptavidin is a biotin-binding protein originally isolated from Streptomyces avidinii. In contrast to avidin, streptavidin has no carbohydrate and has a mildly acidic pI of 5. Thermo Scientific streptavidin products use a recombinant form of streptavidin having a mass of 53,000 daltons and a near-neutral pI. Streptavidin is a tetrameric protein, with each subunit binding one molecule of biotin with affinity similar to that of avidin. Guanidinium chloride will dissociate avidin and streptavidin into subunits, but streptavidin is more resistant to dissociation.

Streptavidin, Alkaline Phosphatase Conjugate enables detection of biotinylated antibodies and other probes in a variety of standard assay methods, including western blotting, ELISA, immunohistochemistry, and fluorescence imaging.

Recommended concentrations: ELISA (0.1 µg/mL), immunofluorescence (assay dependent), western blot (2 µg/mL)

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Label or Dye: AP (Alkaline Phosphatase)
Product Size: 1 mg
Target Molecule: Biotin

Contents & storage

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, but do not store above -20°C.

Documents

Manuals & protocols