Pierce™ Streptavidin

Catalog number: 21145

Thermo Scientific™  Related applications: Cellular Imaging | Protein Assays and Analysis

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Description

Thermo Scientific Pierce Streptavidin is a purified unconjugated recombinant protein form.

Features of Streptavidin:

Recombinant streptavidin—high-quality protein, 53kDa, pI = 6.8 to 7.5
Pure protein —choose pure, unconjugated protein for biotin blocking and controls

The purified recombinant Streptavidin enables detection of biotinylated antibodies and other probes in a variety of standard assay methods, including Western blotting, ELISA, immunohistochemistry and fluorescence imaging. Purified streptavidin is supplied as lyophilized in phosphate-based buffers for immediate reconstitution with water.

Streptavidin is biotin-binding protein that was originally isolated from Streptomyces avidinii. In contrast to avidin, streptavidin has no carbohydrate and has a mildly acidic pI of 5. Thermo Scientific Pierce Streptavidin products use a recombinant form of streptavidin having a mass of 53,000 daltons and a near-neutral pI. Streptavidin is a tetrameric protein, with each subunit binding one molecule of biotin with affinity similar to that of avidin. Guanidinium chloride will dissociate avidin and streptavidin into subunits, but streptavidin is more resistant to dissociation.

Related Products
Pierce™ Streptavidin, Horseradish Peroxidase Conjugated
Pierce™ Streptavidin, Alkaline Phosphatase Conjugated
Pierce™ Streptavidin, Maleimide-Activated
Pierce™ Streptavidin, Hydrazide-Activated
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Label or Dye: Unlabeled
Product Size: 1000 mg
Target Molecule: Biotin

Contents & storage

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2 to 8°C.

Documents

Manuals & protocols