GMBS (N-γ-maleimidobutyryl-oxysuccinimide ester)

Zitierungen und Referenzen (7)

Thermo Scientific™

GMBS (N-γ-maleimidobutyryl-oxysuccinimide ester)

Katalognummer	Menge
22309	50 mg

Katalognummer 22309

Preis (EUR)

230,00

Zum Warenkorb hinzufügen

Menge:

50 mg

Preis (EUR)

230,00

Zum Warenkorb hinzufügen

Thermo Scientific Pierce GMBS ist ein Amin-Sulfhydryl-Crosslinker mit NHS-Ester und Maleimid-reaktive Gruppen an gegenüberliegenden Enden eines kurzen Spacerarms (7,3 Angström).

Merkmale von GMBS:

• Reaktive Gruppen: NHS-Ester und Maleimid
• Reaktiv gegen: Amino- und Sulfhydrylgruppen
• C4-Homologon der aliphatischen Spacer-Serie, die AMAS, BMPS, GMBS und EMCS enthält
• NHS-Ester-Endpaare koppeln mit primären Aminen bei einem pH-Wert von 7 bis 9, um stabile Amid-Bindungen zu bilden
• Maleimide reagieren mit -SH-Gruppen bei einem pH-Wert von 6,5 bis 7,5 und bilden stabiler Thioether-Verbindungen
• Nicht spaltbar
• Wasserunlöslich (zuerst in DMF oder DMSO auflösen); Vergleich mit Sulfo-GMBS
• Kurzer aliphatischer Spacer hat geringes Potenzial für die Hervorrufung einer Immunantwort

Produktreferenz:
Crosslinker Anwendungsleitfaden -- Suchen Sie nach aktuellen Literaturreferenzen für dieses Produkt

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

ZellpermeabilitätJa

BeschreibungGMBS

FormPulver

MarkierungsmethodeChemische Markierung

Molekulargewicht280,23

PEGyliertNein

ProduktliniePierce

Menge50 mg

Reaktiver TeilMaleimid, NHS-Ester

VersandbedingungUmgebungstemperatur

LöslichkeitDMF, DMSO

Länge des Spacer-Arms7,3 Å

WasserlöslichNein

Chemische ReaktivitätAmin-Sulfhydryl

CleavableNein

Crosslinker-TypHeterobifunktional

FormatStandard

ProdukttypCrosslinker

AbstandshalterKurz (<10 Å)

Unit SizeEach

Inhalt und Lagerung

Nach Erhalt getrocknet bei 4 °C lagern.

Häufig gestellte Fragen (FAQ)

Can you provide the shelf-life for GMBS (N-γ-maleimidobutyryl-oxysuccinimide ester)?

GMBS (N-γ-maleimidobutyryl-oxysuccinimide ester) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Zitierungen und Referenzen (7)

Zitierungen und Referenzen

Abstract

Solid phase DNA amplification: characterisation of primer attachment and amplification mechanisms.

Authors:Adessi C, Matton G, Ayala G, Turcatti G, Mermod JJ, Mayer P, Kawashima E

Journal:Nucleic Acids Res

PubMed ID:11024189

Different chemical methods used to attach oligonucleotides by their 5'-end on a glass surface were tested in the framework of solid phase PCR where surface-bound instead of freely-diffusing primers are used to amplify DNA. Each method was first evaluated for its capacity to provide a high surface coverage of oligonucleotides ... More

Covalent attachment of synthetic DNA to self-assembled monolayer films.

Authors:Chrisey LA, Lee GU, O'Ferrall CE

Journal:Nucleic Acids Res

PubMed ID:8760890

'The covalent attachment of thiol-modified DNA oligomers; to self-assembled monolayer silane films on fused silica and oxidized silicon substrates is described. A heterobifunctional crosslinking molecule bearing both thiol- and amino-reactive moieties was used to tether a DNA oligomer (modified at its terminus with a thiol group) to an aminosilane film ... More

Recognition of galactan components of pectin by galectin-3.

Authors:Gunning AP, Bongaerts RJ, Morris VJ

Journal:FASEB J

PubMed ID:18832596

It has been reported that modified forms of pectin possess anticancer activity. To account for this bioactivity, it has been proposed that fragments of pectin molecules can act by binding to and inhibiting the various roles of the mammalian protein galectin 3 (Gal3) in cancer progression and metastasis. Despite this ... More

Mechanism for phosphatidylserine-dependent erythrophagocytosis in mouse liver.

Authors:Lee SJ, Park SY, Jung MY, Bae SM, Kim IS

Journal:Blood

PubMed ID:21427291

Aged or damaged RBCs are effectively removed from the blood circulation by Kupffer cells in the liver, but little is known regarding the mechanism of the clearance process. Here we show that stabilin-1 and stabilin-2 in hepatic sinusoidal endothelial cells (HSECs) are critical in effectively clearing damaged RBCs in mouse ... More

Mapping specific adhesive interactions on living human intestinal epithelial cells with atomic force microscopy.

Authors:Gunning AP, Chambers S, Pin C, Man AL, Morris VJ, Nicoletti C

Journal:FASEB J

PubMed ID:18263697

Specific molecular-receptor interactions with gut epithelium cells are important in understanding bioactivity of food components and drugs, binding of commensal microflora, attachment and initiation of defense mechanisms against pathogenic bacteria and for development of targeted delivery systems to the gut. However, methods for probing such interactions are lacking. Methodology has ... More

View all GMBS (N-γ-maleimidobutyryl-oxysuccinimide ester) citations