GMBS (N-γ-maleimidobutyryl-oxysuccinimide ester)

Citas y referencias (7)

Thermo Scientific™

GMBS (N-γ-maleimidobutyryl-oxysuccinimide ester)

Thermo Scientific Pierce GMBS es un entrecruzador de amina a sulfhidrilo que contiene grupos reactivos de éster NHS y maleimidaMás información

Número de catálogo	Cantidad
22309	50 mg

Número de catálogo 22309

Precio (MXN)

Cantidad:

50 mg

Thermo Scientific Pierce GMBS es un entrecruzador de amina a sulfhidrilo que contiene grupos reactivos de éster NHS y maleimida en los extremos opuestos de un brazo espaciador corto (7,3 angstroms).

Características de GMBS:

• Grupos reactivos: Éster NHS y maleimida
• Reactivo hacia: grupos amino y sulfhidrilo
• C4-homólogo de la serie de espaciadores alifáticos que incluye AMAS, BMPS, GMBS y EMCS
• El extremo de éster NHS se une con aminas primarias a pH 7-9 para formar enlaces de amida estables
• Las maleimidas reaccionan con grupos -SH a pH 6,5-7,5, formando enlaces de tioéter estables
•No escindible
• Insoluble en agua (disolver primero en DMF o DMSO); comparar con Sulfo-GMBS
• El espaciador corto alifático tiene un bajo potencial para obtener una respuesta inmunitaria

Referencias de producto:
Guía de aplicación entrecruzada: búsqueda de referencias literarias recientes para este producto

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Permeabilidad celularSí

DescripciónGMBS

FormularioPolvo

Método de etiquetadoEtiqueta química

Peso molecular280.23

PegiladoNo

Línea de productosPierce

Cantidad50 mg

Fracción reactivaMaleimida, Éster NHS

Condiciones de envíoAmbiente

SolubilidadDMF, DMSO

Longitud del brazo del separador7,3 Å

Soluble en aguaNo

Reactividad químicaAmino-sulfhidrilo

CleavableNo

Tipo de enlace cruzadoHeterobifuncionales

FormatoEstándar

Tipo de productoEntrecruzador

SeparadorCorto (< 10 Å)

Unit SizeEach

Contenido y almacenamiento

Tras su recepción, almacenar desecado a 4 °C.

Preguntas frecuentes

Can you provide the shelf-life for GMBS (N-γ-maleimidobutyryl-oxysuccinimide ester)?

GMBS (N-γ-maleimidobutyryl-oxysuccinimide ester) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Citations & References (7)

Citations & References

Abstract

Solid phase DNA amplification: characterisation of primer attachment and amplification mechanisms.

Authors:Adessi C, Matton G, Ayala G, Turcatti G, Mermod JJ, Mayer P, Kawashima E

Journal:Nucleic Acids Res

PubMed ID:11024189

Different chemical methods used to attach oligonucleotides by their 5'-end on a glass surface were tested in the framework of solid phase PCR where surface-bound instead of freely-diffusing primers are used to amplify DNA. Each method was first evaluated for its capacity to provide a high surface coverage of oligonucleotides ... More

Covalent attachment of synthetic DNA to self-assembled monolayer films.

Authors:Chrisey LA, Lee GU, O'Ferrall CE

Journal:Nucleic Acids Res

PubMed ID:8760890

'The covalent attachment of thiol-modified DNA oligomers; to self-assembled monolayer silane films on fused silica and oxidized silicon substrates is described. A heterobifunctional crosslinking molecule bearing both thiol- and amino-reactive moieties was used to tether a DNA oligomer (modified at its terminus with a thiol group) to an aminosilane film ... More

Recognition of galactan components of pectin by galectin-3.

Authors:Gunning AP, Bongaerts RJ, Morris VJ

Journal:FASEB J

PubMed ID:18832596

It has been reported that modified forms of pectin possess anticancer activity. To account for this bioactivity, it has been proposed that fragments of pectin molecules can act by binding to and inhibiting the various roles of the mammalian protein galectin 3 (Gal3) in cancer progression and metastasis. Despite this ... More

Mechanism for phosphatidylserine-dependent erythrophagocytosis in mouse liver.

Authors:Lee SJ, Park SY, Jung MY, Bae SM, Kim IS

Journal:Blood

PubMed ID:21427291

Aged or damaged RBCs are effectively removed from the blood circulation by Kupffer cells in the liver, but little is known regarding the mechanism of the clearance process. Here we show that stabilin-1 and stabilin-2 in hepatic sinusoidal endothelial cells (HSECs) are critical in effectively clearing damaged RBCs in mouse ... More

Mapping specific adhesive interactions on living human intestinal epithelial cells with atomic force microscopy.

Authors:Gunning AP, Chambers S, Pin C, Man AL, Morris VJ, Nicoletti C

Journal:FASEB J

PubMed ID:18263697

Specific molecular-receptor interactions with gut epithelium cells are important in understanding bioactivity of food components and drugs, binding of commensal microflora, attachment and initiation of defense mechanisms against pathogenic bacteria and for development of targeted delivery systems to the gut. However, methods for probing such interactions are lacking. Methodology has ... More

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