Thermo Scientific Pierce GMBS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a short spacer arm (7.3 angstroms).
Features of GMBS:
• Reactive groups: NHS ester and maleimide • Reactive toward: amino and sulfhydryl groups • C4-homolog of aliphatic spacer series which includes AMAS, BMPS, GMBS and EMCS • NHS ester end couples with primary amines at pH 7-9 to form stable amide bonds • Maleimides react with -SH groups at pH 6.5-7.5, forming stable thioether linkages • Non-cleavable • Water-insoluble (dissolve first in DMF or DMSO); compare to Sulfo-GMBS • Short aliphatic spacer has low potential for eliciting an immune response
Product References: Crosslinker Application Guide -- search for recent literature references for this product
For Research Use Only. Not for use in diagnostic procedures.