AMAS (N-α-maleimidoacet-oxysuccinimide ester)

Citas y referencias (4)

Thermo Scientific™

AMAS (N-α-maleimidoacet-oxysuccinimide ester)

Thermo Scientific Pierce AMAS es un entrecruzador de amina a sulfhidrilo que contiene grupos reactivos de éster NHS y maleimidaMás información

Número de catálogo	Cantidad
22295	50 mg

Número de catálogo 22295

Precio (MXN)

Cantidad:

50 mg

Pedido a granel o personalizado

Thermo Scientific Pierce AMAS es un entrecruzador de amina a sulfhidrilo que contiene grupos reactivos de éster NHS y maleimida en los extremos opuestos de un brazo separador muy corto (4,4 angstroms).

Características de AMAS:

• Grupos reactivos: Éster NHS y maleimida
• Reactivo hacia: grupos amino y sulfhidrilo
• El más corto de la serie de espaciadores alifáticos que incluye AMAS, BMPS, GMBS y EMCS
• El extremo del éster NHS se une con aminas primarias a pH 7-9, formando enlaces de amida estables
• La maleimida reacciona con los grupos -SH a pH 6,5-7,5, formando enlaces de tioéter estables
• No escindible
• Insoluble en agua (disolver primero en DMF o DMSO)
• El espaciador alifático muy corto tiene un bajo potencial para obtener una respuesta inmune

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Permeabilidad celularSí

DescripciónAMAS

FormularioPolvo

Método de etiquetadoEtiqueta química

Peso molecular252.18

PegiladoNo

Línea de productosPierce

Cantidad50 mg

Fracción reactivaMaleimida, Éster NHS

Condiciones de envíoAmbiente

SolubilidadDMF, DMSO

Longitud del brazo del separador4,4 Å

Soluble en aguaNo

Reactividad químicaAmino-sulfhidrilo

CleavableNo

Tipo de enlace cruzadoHeterobifuncionales

FormatoEstándar

Tipo de productoEntrecruzador

SeparadorCorto (< 10 Å)

Unit SizeEach

Contenido y almacenamiento

Tras su recepción, almacenar desecado a 4 °C.

Preguntas frecuentes

Can you provide the shelf-life for AMAS (N-α-maleimidoacet-oxysuccinimide ester)?

AMAS (N-α-maleimidoacet-oxysuccinimide ester is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Citations & References (4)

Citations & References

Abstract

Dual binding mode of the nascent polypeptide-associated complex reveals a novel universal adapter site on the ribosome.

Authors:Pech M, Spreter T, Beckmann R, Beatrix B

Journal:J Biol Chem

PubMed ID:20410297

'Nascent polypeptide-associated complex (NAC) was identified in eukaryotes as the first cytosolic factor that contacts the nascent polypeptide chain emerging from the ribosome. NAC is present as a homodimer in archaea and as a highly conserved heterodimer in eukaryotes. Mutations in NAC cause severe embryonically lethal phenotypes in mice, Drosophila ... More

Analysis of RovA, a transcriptional regulator of Yersinia pseudotuberculosis virulence that acts through antirepression and direct transcriptional activation.

Authors:Tran HJ, Heroven AK, Winkler L, Spreter T, Beatrix B, Dersch P

Journal:J Biol Chem

PubMed ID:16257976

'The transcription factor RovA of Yersinia pseudotuberculosis and analogous proteins in other Enterobacteriaceae activate the expression of virulence genes that play a crucial role in stress adaptation and pathogenesis. In this study, we demonstrate that the RovA protein forms dimers independent of DNA binding, stimulates RNA polymerase, most likely via ... More

A central role for the T1 domain in voltage-gated potassium channel formation and function.

Authors:Strang C, Cushman SJ, DeRubeis D, Peterson D, Pfaffinger PJ

Journal:J Biol Chem

PubMed ID:11312262

To interpret the recent atomic structures of the Kv (voltage-dependent potassium) channel T1 domain in a functional context, we must understand both how the T1 domain is integrated into the full-length functional channel protein and what functional roles the T1 domain governs. The T1 domain clearly plays a role in ... More

Spatial and dynamic interactions between phospholamban and the canine cardiac Ca2+ pump revealed with use of heterobifunctional cross-linking agents.

Authors:Chen Z, Stokes DL, Rice WJ, Jones LR

Journal:J Biol Chem

PubMed ID:12972413

Heterobifunctional thiol to amine cross-linking agents were used to gain new insights on the dynamics and conformational factors governing the interaction between the cardiac Ca2+ pump (SERCA2a) and phospholamban (PLB). PLB is a small protein inhibitor of SERCA2a that reduces enzyme affinity for Ca2+ and thereby regulates cardiac contractility. We ... More