Blocker™ BSA (10X) in TBS

Catalog number: 37520

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Description

Thermo Scientific Blocker BSA in TBS is a ready-to-use, 1X TBS solution of 10X (10%) bovine serum albumin protein for blocking steps in Western blot, ELISA, IHC and nucleic acid detection methods.

Features of Blocker BSA:

Purified protein—10% solutions of high-quality bovine serum albumin; a single purified protein provides fewer chances of cross-reaction with assay components than serum or milk solutions
Convenient—concentrated formulation saves storage space and can be diluted easily to obtain optimal blocking results for specific applications
Easy to use—no waiting for powder to dissolve with this ready-to-dilute liquid concentrate

This blocking buffer is a 10% (w/v) solution of high-quality BSA that is useful for saturating excess protein-binding sites on membranes and microplates in immunoassays. Blocker BSA Buffers are most frequently diluted 10-fold (to 1% BSA) in 1X Tris-buffered saline for initial testing, but other buffer concentrations can be beneficial for specific systems. Blocker BSA is usually more effective than nonfat milk blocking buffers for biotin-avidin systems because it contains a single purified protein that is devoid endogenous biotin.

The purpose of the blocking step in an assay is to improve assay sensitivity by reducing background interference. However, unforeseen cross-reaction of detection reagents with blocking buffers is itself a cause of high background and low signal-to-noise ratios in assay systems. Because individual blocking buffers are not compatible with every system, a variety of blockers in both Tris-buffered saline (TBS) and phosphate-buffered saline (PBS) are available. The best blocking buffer for a specific experiment will bind to all potential sites of nonspecific interaction, eliminating background without altering or obscuring the epitope for antibody binding.

To optimize the blocking step for a particular immunoassay, empirical testing is essential. Using inadequate amounts of blocker will result in excessive background. Using an excessive blocker concentration can mask antibody-antigen interactions or inhibit the marker enzyme. For best results when developing a new immunoassay, test several different blocking agents for the highest signal-to-noise ratio in the assay. There is no single blocking agent that is ideal for every occasion because many factors can influence nonspecific binding, including various protein interactions unique to a specific assay system.

Related Products
Blocker™ BSA (10X) in PBS

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form: Liquid
Product Size: 125 mL
Reagent Type: Blocking Reagent
Validated Application: ELISA, Western Blot

Contents & storage

Store at 4°C.

Documents

Manuals & protocols

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