Invitrogen

beta-3 Tubulin Monoclonal Antibody (2G10)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

10 Published Figures

24 References

View all (48) Beta III Tubulin antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

beta-3 Tubulin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 24

beta-3 Tubulin Antibody (MA1-118) in ICC/IF

Immunofluorescence analysis of beta III tubulin (green) in the ectoderm derived from human ES cells. Embryoid bodies (EBs) were generated from the H9 embryonic stem cell line (WiCell Research Institute, WA09) using Gibco® KnockOut™ Serum Replacement. After four days in suspension culture, EBs were plated on Geltrex™-coated tissue culture-treated polystyrene and continuously cultured for 21 days. EB cultures... View More

Product Image Gallery

beta-3 Tubulin Antibody in Western Blot (WB) — Western blot analysis of beta-3 Tubulin was performed by loading 25 µg of various brain, SHSY5Y, and HeLa cell lysates and 10 µL of PageRuler Prestained Protein Ladder (Product # 26616) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a beta-3 Tubulin monoclonal antibody (Product # MA1-118) at a dilution of 1:2000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti-mouse IgG-HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080). Note: analysis indicates both reactive (neuronal) and non-reactive negative control (HeLa cell lysate) for beta-3 Tubulin using Product # MA1-118.

beta-3 Tubulin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on normal biopsies of deparaffinized human brain tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer for 20 minutes at 95°C. Following antigen retrieval, tissues were blocked in 2% Blocker BSA (Product # 37525) in PBST for 1 hour at room temperature and then probed without (left panel) or with (right panel) a beta-3 Tubulin monoclonal antibody (Product # MA1-118) at a dilution of 1:100 for 1 hour at room temperature in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity quenched with Peroxidase Suppressor (Product # 35000) for 30 minutes at room temperature. Detection was performed using an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:500 followed by colorimetric detection using metal enhanced DAB. Tissues were counterstained with hematoxylin and prepped for mounting. Images were taken on a Zeiss Axiovision microscope at 32X magnification.

beta-3 Tubulin Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunofluorescent analysis of beta-3 Tubulin (green), PAX6 (red), and CTIP2 (blue) in human iPSC-derived forebrain organoids derived at Day 40. The organoids were fixed with 4% PFA for 1 hour at room temperature, followed by incubation with 30% sucrose solution overnight at 4°C. The organoids were then embedded in OCT and cryosectioned at 5 µm, permeabilized with 0.2% Triton X-100 for 20 min, and blocked with 10% donkey serum in PBS for 30 min at room temperature. Organoid slices were stained with a Mouse beta-3 Tubulin monoclonal antibody (green; Product # MA1-118) at a dilution of 1:500, a Rabbit PAX6 polyclonal antibody (red; Product # 42-6600) at a dilution of 1:500, and a Rat CTIP2 polyclonal antibody (blue) at a dilution of 1:300 in blocking buffer overnight at 4°C, and then incubated with Donkey anti-Mouse Alexa Fluor 488 (Product # R37114), Donkey anti-Rabbit Alexa Fluor 568 (Product # A10042), and Goat anti-Rat Alexa Fluor 350 (Product # A-21093) at a dilution of 1:1000 in blocking solution at room temperature for 1 hour. Images were taken at 20X magnification. Data courtesy of Dr. Zhexing Wen at Emory University.

beta-3 Tubulin Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of beta-3 Tubulin (blue histogram) on human SHSY5Y neuroblast cells. Cells were harvested, fixed with 4% formaldehyde, washed with PBS, and incubated with a beta-3 Tubulin monoclonal antibody (Product # MA1-118) at a 1:50 dilution or PBS alone (red histogram) for 1 hour at room temperature. For flow cytometry analysis, a 30-minute incubation with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) was performed and 10,000 cells were acquired for each sample.

beta-3 Tubulin Antibody in Immunoprecipitation (IP) — Immunoprecipitation of beta-3 Tubulin was performed using SHSY5Y whole cell lysate. Antigen-antibody complexes were formed by incubating 300 µg of lysate with 5 µg of a beta-3 Tubulin monoclonal antibody (Product # MA1-118) overnight on a rocking platform at 4°C. The immune complexes were captured on 50 µL Protein A/G Agarose (Product # 20421), washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer (Product # 39000). SHSY5Y cell lysate (30 µg) was loaded as a positive control (left lane). The sample was resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a beta-3 Tubulin monoclonal antibody (Product # MA1-118) at a dilution of 1:1000 overnight rotating at 4°C, washed in TBST, and probed with Clean-blot IP Detection Reagent (Product # 21230) at a dilution of 1:1000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).

beta-3 Tubulin Antibody in Immunohistochemistry (IHC) — Fig. 6 SARS-CoV-2 antigens detection and cytokine/chemokine transcripts quantification in the olfactory system of hamsters. ( A-D ) Olfactory epithelium of mock- ( A, C ) and SARS-CoV-2 ( B, D ) infected hamsters at 4 dpi. Insets show infected OMP + mature olfactory sensory neurons ( B ), or infected Tuj1 + immature olfactory sensory neurons ( D ). The arrow in D indicates an infected Iba1+ cell. ( E ) Sagittal section showing nasal turbinates and olfactory bulb of SARS-CoV-2 infected hamster at 4 dpi. Inset depicts SARS-CoV-2 staining in olfactory sensory neuron axons. ( F ) Olfactory sensory axons projecting into glomeruli in the olfactory bulb of SARS-CoV-2 inoculated hamsters at 4 dpi. Insets ( F', F'' ) show infected cells. ( G-I ) Olfactory bulb of mock-( G ) or SARS-CoV-2 ( H, I ) infected hamsters at 4 dpi. Iba1 + infected cells are shown in ( H ) and several infected cells are observed in ( I ). SARS-CoV-2 is detected by antibodies raised against the viral nucleoprotein (NP). Scale bars: 20mum (A-D, H), 100mum (E, F), 50mum (G, I). Images are single z-planes (A-H) or maximum intensity projection over a 6mum depth (I). ( J ) Number of NP+ cells, NP+OMP+ cells, NP+Tuj1+ cells, NP+Iba1+, Iba1+ cells and cleaved caspase 3+ cells in the olfactory mucosa. ( K, L ) Number of NP+ cells in the olfactory nerve (K) and the olfactory bulb (L). ( M, N ) Cytokines and chemokines transcripts in the nasal turbinates ( M ) and in the olfactory bulb ( N ) at 2, 4 and 14 dpi. Mann-Whit

beta-3 Tubulin Antibody — The specificity of mouse anti-Beta-3-Tubulin monoclonal antibody (Product # MA1-118) was demonstrated in the immunofluorescence analysis the detection of increased expression of Beta-3-Tubulin in SHSY5Y cells upon treatment with 10 uM Retinoic acid compared to untreated cells. {TM}

Product Details

MA1-118

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

Assay-dependent

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:50-1:200

View 12 publications 12 publications

Flow Cytometry (Flow)

1:50

Immunoprecipitation (IP)

5 µg

Product Specifications

Species Reactivity

Bovine, Guinea pig, Hamster, Human, Mouse, Pig, Rabbit, Rat

Published species

Amphibian, Human, Mouse, Rabbit, Rat, Zebrafish

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

2G10

Immunogen

A synthetic peptide corresponding to amino acids 436-450 from rat neuronal specific beta-3 tubulin.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

RRID

AB_2536829

Product Specific Information

MA1-118 was successfully used to detect E18 Sparague Dawley primary cortical neurons.

MA1-118 can be used for immunofluorescence analysis of beta III tubulin in the ectoderm derived from human embryonic stem cells.

Western blot analysis of MA1-118 detects an ~50 kDa protein in neuronal-type cells. In bovine, a unknown band at ~32 kDa is also detected. MA1-118 shows specificity to beta-3 Tubulin and is non-reactive to lysates from non-neuronal cell types (e.g. HeLa cell lysate).

Target Information

The betaIII-tubulin isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CREB3L2-ATF4 heterodimerization defines a transcriptional hub of Alzheimer's disease gene expression linked to neuropathology.

Science advances

Gouveia Roque C,Chung KM,McCurdy EP,Jagannathan R,Randolph LK,Herline-Killian K,Baleriola J,Hengst U

MA1-118 was used in Western Blotting to reveal differential transcription factor dimerization as a mechanism linking disease stimuli to the development of pathogenic cellular states.

Fri Mar 03 00:00:00 EST 2023

Mechanisms and treatments of neuropathic itch in a mouse model of lymphoma.

The Journal of clinical investigation

Chen O,He Q,Han Q,Furutani K,Gu Y,Olexa M,Ji RR

MA1-118 was used in Immunohistochemistry to suggest distinct mechanisms underlying acute, chronic, and neuropathic itch.

Wed Feb 15 00:00:00 EST 2023

A Gelatin Methacrylate-Based Hydrogel as a Potential Bioink for 3D Bioprinting and Neuronal Differentiation.

Pharmaceutics

Cruz EM,Machado LS,Zamproni LN,Bim LV,Ferreira PS,Pinto LA,Pessan LA,Backes EH,Porcionatto MA

MA1-118 was used in Immunocytochemistry-immunofluorescence to indicate that bioinks, 2.5/2.5G/GMA_Gx and 4GMA_Gx, can support neural cell survival and modulate neural differentiation.

Mon Feb 13 00:00:00 EST 2023

Deposition chamber technology as building blocks for a standardized brain-on-chip framework.

Microsystems & nanoengineering

Maisonneuve BGC,Libralesso L,Miny L,Batut A,Rontard J,Gleyzes M,Boudra B,Viera J,Debis D,Larramendy F,Jost V,Honegger T

Published figure using beta-3 Tubulin monoclonal antibody (Product # MA1-118) in Immunocytochemistry

Fri Aug 05 00:00:00 EDT 2022

Modulation of Amyloid β-Induced Microglia Activation and Neuronal Cell Death by Curcumin and Analogues.

International journal of molecular sciences

De Lorenzi E,Franceschini D,Contardi C,Di Martino RMC,Seghetti F,Serra M,Bisceglia F,Pagetta A,Zusso M,Belluti F

Published figure using beta-3 Tubulin monoclonal antibody (Product # MA1-118) in Immunocytochemistry

Fri Apr 15 00:00:00 EDT 2022

Science translational medicine

de Melo GD,Lazarini F,Levallois S,Hautefort C,Michel V,Larrous F,Verillaud B,Aparicio C,Wagner S,Gheusi G,Kergoat L,Kornobis E,Donati F,Cokelaer T,Hervochon R,Madec Y,Roze E,Salmon D,Bourhy H,Lecuit M,Lledo PM

Published figure using beta-3 Tubulin monoclonal antibody (Product # MA1-118) in Immunocytochemistry

Wed Jun 02 00:00:00 EDT 2021

Science translational medicine

Published figure using beta-3 Tubulin monoclonal antibody (Product # MA1-118) in Immunocytochemistry

Wed Jun 02 00:00:00 EDT 2021

Science translational medicine

Published figure using beta-3 Tubulin monoclonal antibody (Product # MA1-118) in Immunocytochemistry

Wed Jun 02 00:00:00 EDT 2021

Neuron and astrocyte aggregation and sorting in three-dimensional neuronal constructs.

Communications biology

Hasan MF,Berdichevsky Y

MA1-118 was used in Immunocytochemistry-immunoflourescence to develop linear, adhered rat and human 3D neuronal-astrocyte cultures to study aggregation and sorting of these cells.

Mon May 17 00:00:00 EDT 2021

Astrocytes Exhibit a Protective Role in Neuronal Firing Patterns under Chemically Induced Seizures in Neuron-Astrocyte Co-Cultures.

International journal of molecular sciences

Ahtiainen A,Genocchi B,Tanskanen JMA,Barros MT,Hyttinen JAK,Lenk K

MA1-118 was used in Immunocytochemistry-immunofluorescence to assess the modulatory roles of astrocytes during epileptic-like conditions and in compensating drug-elicited hyperactivity.

Thu Nov 25 00:00:00 EST 2021

Zika Virus Targets Glioblastoma Stem Cells through a SOX2-Integrin α_vβ₅ Axis.

Cell stem cell

Zhu Z,Mesci P,Bernatchez JA,Gimple RC,Wang X,Schafer ST,Wettersten HI,Beck S,Clark AE,Wu Q,Prager BC,Kim LJY,Dhanwani R,Sharma S,Garancher A,Weis SM,Mack SC,Negraes PD,Trujillo CA,Penalva LO,Feng J,Lan Z,Zhang R,Wessel AW,Dhawan S,Diamond MS,Chen CC,Wechsler-Reya RJ,Gage FH,Hu H,Siqueira-Neto JL,Muotri AR,Cheresh DA,Rich JN

MA1-118 was used in Immunohistochemistry-immunofluorescence to demonstrate that ZIKV preferentially infected and killed GSCs and stem-like cells in medulloblastoma and ependymoma in a SOX2-dependent manner.

Thu Feb 06 00:00:00 EST 2020

P-Glycoprotein Inhibition Exacerbates Paclitaxel Neurotoxicity in Neurons and Patients With Cancer.

Clinical pharmacology and therapeutics

Stage TB,Mortensen C,Khalaf S,Steffensen V,Hammer HS,Xiong C,Nielsen F,Poetz O,Svenningsen ÅF,Rodriguez-Antona C,Kroetz DL

Published figure using beta-3 Tubulin monoclonal antibody (Product # MA1-118) in Immunocytochemistry

Tue Sep 01 00:00:00 EDT 2020

P-Glycoprotein Inhibition Exacerbates Paclitaxel Neurotoxicity in Neurons and Patients With Cancer.

Clinical pharmacology and therapeutics

Stage TB,Mortensen C,Khalaf S,Steffensen V,Hammer HS,Xiong C,Nielsen F,Poetz O,Svenningsen ÅF,Rodriguez-Antona C,Kroetz DL

Published figure using beta-3 Tubulin monoclonal antibody (Product # MA1-118) in Immunocytochemistry

Tue Sep 01 00:00:00 EDT 2020

Modeling medulloblastoma in vivo and with human cerebellar organoids.

Nature communications

Ballabio C,Anderle M,Gianesello M,Lago C,Miele E,Cardano M,Aiello G,Piazza S,Caron D,Gianno F,Ciolfi A,Pedace L,Mastronuzzi A,Tartaglia M,Locatelli F,Ferretti E,Giangaspero F,Tiberi L

Published figure using beta-3 Tubulin monoclonal antibody (Product # MA1-118) in Immunofluorescence

Wed Jan 29 00:00:00 EST 2020

TFAP2C facilitates somatic cell reprogramming by inhibiting c-Myc-dependent apoptosis and promoting mesenchymal-to-epithelial transition.

Cell death & disease

Wang Y,Chen S,Jiang Q,Deng J,Cheng F,Lin Y,Cheng L,Ye Y,Chen X,Yao Y,Zhang X,Shi G,Dai L,Su X,Peng Y,Deng H

MA1-118 was used in Immunocytochemistry to demonstrate Tfap2c is induced during the generation of iPSCs from mouse fibroblasts and acts as a facilitator for iPSCs formation.

Thu Jun 25 00:00:00 EDT 2020

Enhancement of BDNF Expression and Memory by HDAC Inhibition Requires BET Bromodomain Reader Proteins.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Sartor GC,Malvezzi AM,Kumar A,Andrade NS,Wiedner HJ,Vilca SJ,Janczura KJ,Bagheri A,Al-Ali H,Powell SK,Brown PT,Volmar CH,Foster TC,Zeier Z,Wahlestedt C

MA1-118X was used in Immunocytochemistry-immunoflourescence to reveal that BRD4 is necessary for enhancing brain-derived neurotrophic factor (BDNF) expression and improved memory following histone deacetylase inhibition.

Wed Jan 23 00:00:00 EST 2019

Induced Pluripotent Stem Cell Derivation and Ex Vivo Gene Correction Using a Mucopolysaccharidosis Type 1 Disease Mouse Model.

Stem cells international

Miki T,Vazquez L,Yanuaria L,Lopez O,Garcia IM,Ohashi K,Rodriguez NS

MA1-118 was used in Immunocytochemistry-immunoflourescence to support the concept of ex vivo gene editing therapy using iPSC and CRISPR/Cas9 technologies for MPS-1 patients.

Wed Sep 30 00:00:00 EDT 2020

Investigating Nrg1 Signaling in the Regenerating Axolotl Spinal Cord Using Multiplexed FISH.

Developmental neurobiology

Freitas PD,Lovely AM,Monaghan JR

Published figure using beta-3 Tubulin monoclonal antibody (Product # MA1-118) in Immunocytochemistry

Wed May 01 00:00:00 EDT 2019

Investigating Nrg1 Signaling in the Regenerating Axolotl Spinal Cord Using Multiplexed FISH.

Developmental neurobiology

Freitas PD,Lovely AM,Monaghan JR

Published figure using beta-3 Tubulin monoclonal antibody (Product # MA1-118) in Immunocytochemistry

Wed May 01 00:00:00 EDT 2019

Altered Gut Microbiome in Parkinson's Disease and the Influence of Lipopolysaccharide in a Human α-Synuclein Over-Expressing Mouse Model.

Frontiers in neuroscience

Gorecki AM,Preskey L,Bakeberg MC,Kenna JE,Gildenhuys C,MacDougall G,Dunlop SA,Mastaglia FL,Akkari PA,Koengten F,Anderton RS

MA1-118 was used in Western Blotting to show lipopolysaccharides administration in mice causes earlier motor troubles and also an altered gut microbiome is present in patients with Parkinson's disease.

Tue Sep 29 00:00:00 EDT 2020

Growth Cone Tctp Is Dynamically Regulated by Guidance Cues.

Frontiers in molecular neuroscience

Gouveia Roque C,Holt CE

MA1-118 was used in Western Blotting to study the dynamic regulation of growth cone translationally controlled tumour protein by Netrin-1 and Ephrin-A1.

Sat Jan 29 00:00:00 EST 2022

Mesenchymal Stem Cells (MSCs) Coculture Protects [Ca²⁺]_i Orchestrated Oxidant Mediated Damage in Differentiated Neurons In Vitro.

Cells

Alhazzani A,Rajagopalan P,Albarqi Z,Devaraj A,Mohamed MH,Al-Hakami A,Chandramoorthy HC

MA1-118 was used in Immunocytochemistry-immunoflourescence to study the sequence of events that occur during stress and how mesenchymal stem cells complement the rescue of neuronal cell death mediated by [Ca2+]i and reactive oxygen species.

Thu Dec 06 00:00:00 EST 2018

Tubulin Beta-3 Chain as a New Candidate Protein Biomarker of Human Skin Aging: A Preliminary Study.

Oxidative medicine and cellular longevity

Lehmann SG,Bourgoin-Voillard S,Seve M,Rachidi W

Published figure using beta-3 Tubulin monoclonal antibody (Product # MA1-118) in Western Blot

Mon Mar 19 00:00:00 EDT 2018

Sustained Subconjunctival Delivery of Infliximab Protects the Cornea and Retina Following Alkali Burn to the Eye.

Investigative ophthalmology & visual science

Zhou C,Robert MC,Kapoulea V,Lei F,Stagner AM,Jakobiec FA,Dohlman CH,Paschalis EI

MA1-118 was used in immunohistochemistry - frozen section to evaluate the efficacy of subconjunctival delivery of TNF-alpha antibodies using a polymer-based drug delivery system

Sun Jan 01 00:00:00 EST 2017

Mycolactone displays anti-inflammatory effects on the nervous system.

PLoS neglected tropical diseases

Isaac C,Mauborgne A,Grimaldi A,Ade K,Pohl M,Limatola C,Boucher Y,Demangel C,Guenin-Macé L

Published figure using beta-3 Tubulin monoclonal antibody (Product # MA1-118) in Immunofluorescence

Wed Nov 01 00:00:00 EDT 2017

Mycolactone displays anti-inflammatory effects on the nervous system.

PLoS neglected tropical diseases

Isaac C,Mauborgne A,Grimaldi A,Ade K,Pohl M,Limatola C,Boucher Y,Demangel C,Guenin-Macé L

Published figure using beta-3 Tubulin monoclonal antibody (Product # MA1-118) in Immunofluorescence

Wed Nov 01 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: beta 3 tubulin; beta tubulin 3; betaIII-tubulin; class III beta-tubulin; MC1R; melanocortin receptor 1; Neuron specific beta III Tubulin; Neuron-specific class III beta-tubulin; TUBB; tubulin beta; Tubulin beta-3; Tubulin beta-3 chain; Tubulin beta-4; Tubulin beta-4 chain; Tubulin beta-III; tubulin, beta 3 class III; tubulin, beta, 3; tubulin, beta, 4

Gene Aliases: 3200002H15Rik; beta-4; CDCBM; CDCBM1; CFEOM3; CFEOM3A; FEOM3; I79_000194; M(beta)3; M(beta)6; mc1R; TUBB3; TUBB4

UniProt ID: (Bovine) Q2T9S0, (Human) Q13509, (Mouse) Q9ERD7, (Rat) Q4QRB4

Entrez Gene ID: (Rabbit) 100341581, (Bovine) 768070, (Human) 10381, (Mouse) 22152, (Rat) 246118, (Chinese hamster) 100760055

Molecular Function: tubulin