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Invitrogen
RELM alpha Polyclonal Antibody, PeproTech®
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RELM alpha Antibody (500-P214-100UG) in WB
Western Blot: To detect Murine RELMalpha by Western Blot analysis RELM alpha Polyclonal Antibody (Product # 500-P214-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine RELMalpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
500-P214-100UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Human,
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine RELMalpha
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: MDETIEIIVE NKVKELLANP ANYPSTVTKT LSCTSVKTMN RWASCPAGMT ATGCACGFAC GSWEIQSGDT CNCLCLLVDW TTARCCQLS.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine RELMalpha. Anti-Murine RELMalpha-specific antibody was purified by affinity chromatography employing an immobilized Murine RELMalpha matrix.
Sandwich ELISA: To detect Murine RELMalpha by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine RELMalpha (500-P214Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine RELMalpha.
Western Blot: To detect Murine RELMalpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine RELMalpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
RELM alpha, also known as Resistin-like alpha or FIZZ1, is a small cytokine (88 aminoacids, MW 9.4 kDa) produced mostly by macrophages. Its highest constitutive expression has been observed in small peritoneal macrophages, alveolar macrophages and adipose tissue macrophages. It is strongly induced by IL-4 and IL-13. Together with Arginase 1 and Ym-1, RELM alpha is considered one of the hallmarks of the M2 polarization of mouse macrophages, however, macrophages polarized with IL-10 (M2c) express neither RELM alpha nor Arginase 1. Also, the expression patterns of RELM alpha and Arginase 1 in untreated tissue macrophages significantly differ. Elevated levels of RELM alpha may indicate parasitic infection. Humans do not have RELM alpha gene but human Resistin resembles mouse RELM alpha in respect to sequence, tissue distribution and function. Other members of RELM family in mouse: RELM beta, RELM gamma and Resistin display different tissue distribution and functional properties. RELM alpha plays an important role in parasite infection, mucosal inflammation and allergy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cysteine-rich secreted protein A12-gamma; cysteine-rich secreted protein FIZZ1; FIZZ2; found in inflammatory zone 1; HXCP2; hypoxia-induced mitogenic factor; parasite-induced macrophage novel gene 1 protein; PMNG1; FIZZ1; RELM-beta; RELMalpha; FIZZ1; RELMb; RELMbeta; similar to cysteine-rich secreted A12-alpha protein; unnamed protein product
Gene Aliases: 1810019L16Rik; Fizz-1; Fizz1; HIMF; RELM-alpha; RELMa; RELMalpha; Xcp2
UniProt ID: (Mouse) Q9EP95
Entrez Gene ID: (Mouse) 57262
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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