Extracellular plasma RNA from colon cancer patients is confined in a vesicle-like structure and is mRNA-enriched.
AuthorsGarcía JM, García V, Peña C, Domínguez G, Silva J, Diaz R, Espinosa P, Citores MJ, Collado M, Bonilla F
JournalRNA
PubMed ID18456845
'Little is yet known about the origin and protective mechanism of free nucleic acids in plasma. We investigated the possibility of these free nucleic acids being particle associated. Plasma samples from colon cancer patients and cell culture media were subjected to various antibody incubations, ultracentrifugation, and RNA extraction protocols for ... More
Relationships and differentially expressed genes among pancreatic cancers examined by large-scale serial analysis of gene expression.
AuthorsRyu B, Jones J, Blades NJ, Parmigiani G, Hollingsworth MA, Hruban RH, Kern SE
JournalCancer Res
PubMed ID11830538
'Pancreatic adenocarcinoma is among the most fatal of cancers, in part because of late diagnosis and a lack of effective therapies. Comprehensive studies are needed to better understand and address the cellular mechanisms and pathways of tumorigenesis. Serial analysis of gene expression was used to analyze gene expression profiles of ... More
Stem cell and epithelial-mesenchymal transition markers are frequently overexpressed in circulating tumor cells of metastatic breast cancer patients.
AuthorsAktas B, Tewes M, Fehm T, Hauch S, Kimmig R, Kasimir-Bauer S
JournalBreast Cancer Res
PubMed ID19589136
The persistence of circulating tumor cells (CTC) in breast cancer patients might be associated with stem cell like tumor cells which have been suggested to be the active source of metastatic spread in primary tumors. Furthermore, these cells also may undergo phenotypic changes, known as epithelial-mesenchymal transition (EMT), which allows ... More
The transcription factors SOX9 and SOX10 are vitiligo autoantigens in autoimmune polyendocrine syndrome type I.
AuthorsHedstrand H, Ekwall O, Olsson MJ, Landgren E, Kemp EH, Weetman AP, Perheentupa J, Husebye E, Gustafsson J, Betterle C, Kämpe O, Rorsman F
JournalJ Biol Chem
PubMed ID11423552
Vitiligo is common in the hereditary disorder autoimmune polyendocrine syndrome type I (APS I). Patients with APS I are known to have high titer autoantibodies directed against various tissue-specific antigens. Using sera from APS I patients for immunoscreening of a cDNA library from human scalp, we identified the transcription factors ... More
Disease-associated mutations in human mannose-binding lectin compromise oligomerization and activity of the final protein.
AuthorsLarsen F, Madsen HO, Sim RB, Koch C, Garred P
JournalJ Biol Chem
PubMed ID14764589
Deficiency of human mannose-binding lectin (MBL) caused by mutations in the coding part of the MBL2 gene is associated with increased risk and severity of infections and autoimmunity. To study the biological consequences of MBL mutations, we expressed wild type MBL and mutated MBL in Chinese hamster ovary cells. The ... More
Reprogramming of the paternal genome upon fertilization involves genome-wide oxidation of 5-methylcytosine.
AuthorsIqbal K, Jin SG, Pfeifer GP, Szabó PE
JournalProc Natl Acad Sci U S A
PubMed ID21321204
Genome-wide erasure of DNA cytosine-5 methylation has been reported to occur along the paternal pronucleus in fertilized oocytes in an apparently replication-independent manner, but the mechanism of this reprogramming process has remained enigmatic. Recently, considerable amounts of 5-hydroxymethylcytosine (5hmC), most likely derived from enzymatic oxidation of 5-methylcytosine (5mC) by TET ... More