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Invitrogen
Multispecies AKT (Phospho) [pS473] InstantOne™ ELISA Kit
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![Multispecies AKT (Phospho) [pS473] InstantOne™ ELISA Kit](https://www.thermofisher.com/antibody/images/650/akt-phospho-tds.jpg?time=20240507 "Multispecies AKT (Phospho) [pS473] InstantOne™ ELISA Kit")
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Multispecies AKT (Phospho) [pS473] InstantOne™ ELISA Kit
Representative data for AKT (Phospho) [pS473] Multispecies InstantOne™ ELISA. MCF7 cells were pre-treated with 10 µM wortmannin washed and unstimulated (-) or stimulated with 10 ng/mL insulin for 10 min (+). Cells were analyzed by immunoblotting (left) and InstantOne™ ELISA (right). The intensity of the band at 56 kDa as detected by immunoblot correlates with InstantOne™ ELISA absorption values.
![Multispecies AKT (Phospho) [pS473] InstantOne™ ELISA Kit](https://www.thermofisher.com/antibody/images/150/akt-phospho-tds.jpg?time=20240507)
![Multispecies AKT (Phospho) [pS473] InstantOne™ ELISA Kit (85-86042-103)](https://www.thermofisher.com/antibody/images/150/TGR.ELISA.Kit-650x600.jpg?time=20240507)
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Product Specifications
Sample type/volume
Cell Lysate
20 µL
Hands-on time
1 hr 20 min
Time-to-result
1 hr 10 min
Homogenous (no wash)
No
Instrument
Absorbance Microplate Reader
Product size
10 x 384 Tests
Contents
Capture Antibody
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
AKT1
Target site-specificity
Phosphorylated Ser473
Protein subtype
Adaptor Proteins
Species (tested)
Human
Assay kit format
InstantOne ELISA
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human, mouse, and hamster AKT 1/2/3 in cell lysates. Detection of rat AKT 1/2/3 is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.
Target information
AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : AKT, AKT1, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
Gene ID : (Human) 207
Gene symbol : AKT1
Protein Aliases : AKT, AKT1M, CAKT, C-AKT, PKB, PKB ALPHA, RAC-PK-gamma, V-AKT MURINE THYMOMA VIRAL ONCOGENE HOMOLOG 1, V-AKT MURINE THYMOMA VIRAL ONCOGENE-LIKE PROTEIN 1, PROTO-ONCOGENE C-AKT, RAC, RAC-ALPHA, RAC-ALPHA SERINE/THREONINE-PROTEIN KINASE, RAC-PK-ALPHA, RAC-PK-beta, PKB beta, PKB gamma, PKB-ALPHA, PKBG, PRKBA, PROTEIN KINASE B ALPHA, AKT1m, PKB alpha, Protein kinase B, Protein kinase B alpha, Proto-oncogene c-Akt, rac protein kinase alpha, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha, serine-threonine protein kinase, unnamed protein product, v-akt murine thymoma viral oncogene homolog 1, v-akt murine thymoma viral oncogene-like protein 1
UniProt ID (Human) P31749
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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