Thermo Scientific™


Catalog number:  88902

Related applications:

Protein Labeling & Crosslinking

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Thermo Scientific NHS-Azide is an amine-reactive compound that can be used to derivatize primary amines of proteins or amine-coated polymer surfaces for ligation to phosphine-modified molecules.

This NHS-ester compound reacts to form covalent bonds with primary amines (e.g., side chain of lysine residues or aminosilane-coated surfaces). The azide (N3) group reacts with phosphine-labeled molecules by a mechanism known as Staudinger chemistry, enabling efficient and specific conjugation of derivatized molecules in biological samples.

Features of NHS-Azide:

Soluble—reagent easily dissolves in water-miscible solvents for subsequent dilution in aqueous reaction mixtures with cell lysates and other biological samples
Compatible—reaction chemistry occurs effectively in simple buffer conditions; requires no accessory reagents such as copper or reducing agents
Specific—NHS esters are specific for covalent attachment to primary amines (side chain of lysine or N-terminus of polypeptides)
Chemoselective—azide and phosphine groups do not react or interfere with components of biological samples (bioorthogonal) but conjugate to one another with high efficiency

Related Products
For Research Use Only. Not for use in diagnostic procedures.


Product Line: Pierce™
Crosslinker Type: Heterobifunctional
Labeling Method: Chemical Labeling
Reactive Moiety: Amine, Azide, NHS Ester
Solubility: DMSO (Dimethylsulfoxide), DMF (Dimethylformamide)
Spacer: Short
Description: Pierce NHS-Azide

Contents & storage

Upon receipt store at -20°C protected from light and moisture. Product is shipped at ambient temperature.