Thermo Scientific™

NHS-Azide

Catalog number:  88902

Related applications:

Protein Biology

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Description

Thermo Scientific NHS-Azide is an amine-reactive compound that can be used to derivatize primary amines of proteins or amine-coated polymer surfaces for ligation to phosphine-modified molecules.

This NHS-ester compound reacts to form covalent bonds with primary amines (e.g., side chain of lysine residues or aminosilane-coated surfaces). The azide (N3) group reacts with phosphine-labeled molecules by a mechanism known as Staudinger chemistry, enabling efficient and specific conjugation of derivatized molecules in biological samples.

Features of NHS-Azide:

Soluble—reagent easily dissolves in water-miscible solvents for subsequent dilution in aqueous reaction mixtures with cell lysates and other biological samples
Compatible—reaction chemistry occurs effectively in simple buffer conditions; requires no accessory reagents such as copper or reducing agents
Specific—NHS esters are specific for covalent attachment to primary amines (side chain of lysine or N-terminus of polypeptides)
Chemoselective—azide and phosphine groups do not react or interfere with components of biological samples (bioorthogonal) but conjugate to one another with high efficiency

Related Products
NHS-PEG4-Azide
NHS-Phosphine
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Line: Pierce™
Crosslinker Type: Heterobifunctional
Labeling Method: Chemical Labeling
Reactive Moiety: Amine, Azide, NHS Ester
Solubility: DMSO (Dimethylsulfoxide), DMF (Dimethylformamide)
Spacer: Short
Description: Pierce NHS-Azide

Contents & storage

Upon receipt store at -20°C protected from light and moisture. Product is shipped at ambient temperature.