Invitrogen™

PIK3CA E545K/PIK3R1 (p110 alpha E545K/p85 alpha) Recombinant Human Protein

Catalog number:  A33394

 Related applications:

Pharma & Biopharma

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Description

This PIK3CA E545K/PIK3R1 (p110 alpha E545K/p85 alpha) recombinant human protein (full length) was expressed in insect cells. PIK3CA (E545K)/PIK3R1 lipid kinase (p110 alpha E545K/p85 alpha) consists of a 110 kDa catalytic subunit and an 85 kDa regulatory subunit. A number of isoforms of the 110 kDa catalytic subunit and the 85 kDa regulatory subunit exist in cells. The PIK3CA catalytic subunit is frequently mutated or amplified in a variety of cancers including ovarian and colon and this protein is one of the PI3K mutants. PIK3CA gene copy number is increased in over 30% of ovarian cancers and this leads to increased PI3-kinase activity. Furthermore, the activity of PIK3CA is essential for vascular development and inactivation of PIK3Ca leads to severe defects in angiogenic sprouting and vascular remodeling. PIK3CA E545K/PIK3R1 (p110 alpha E545K/p85 alpha) recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the Adapta™ assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Accession Number: PIK3CA E455K: NP_006209.2, PIK3R1(p110a) : NP_852664.1
Expression: Insect
Molecular Weight: 125.4/84.7 kDa
Product Size: 1 mg
Protein Length or Portion (a.a.): Full Length
Protein Tag or Fusion: His Tag
Validated Application: Kinase Assay
Kinase Family: PI3 Kinase
Protein Form: Active, Full Length, Mutant, Recombinant
Shipping Condition: Dry Ice

Contents & storage

Storage Buffer: 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol.

Documents

Manuals & protocols