ABsolute QPCR Mix, no ROX

Catalog number: AB1132B

Thermo Scientific™  Related applications: Real Time PCR (qPCR)

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Thermo Scientific ABsolute qPCR Mixes are optimized for use with hybridization probes and contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes. The 2X master mix contains a proprietary reaction buffer that provides high sensitivity, specificity, and reproducibility. ABsolute qPCR Mixes are available with the option of included high ROX reference dye concentration, Low ROX concentration, and with no ROX reference dye. There is also a master mix version optimized for capillary instruments.


• High sensitivity & specificity - The master mixes contain our patented hot start enzyme Thermo-Start DNA Polymerase
• Reproducibility - All mixes undergo extensive QC to ensure batch to batch consistency
• Optimised buffer - For maximal performance in multiplex reactions

• Gene expression analysis
• siRNA validation
• Genotyping

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For Research Use Only. Not for use in diagnostic procedures.


Detection Method: Primer-Probe Detection
For Use With (Equipment): Cepheid SmartCycler, BioRad iQ5, MJ Chromo4, Corbett RotorGene, MJ Opticon, Bio-Rad CFX384 Touch™, Eppendorf MasterCycler RealPlex, Roche LightCycler 480, BioRad CFX96™, BioRad MyiQ, BioRad iCycler iQ
Reaction Speed: Standard
PCR Method: qPCR
Sample Type (General): dsDNA, DNA
Polymerase: Taq DNA Polymerase
Product Size: 1,600 reactions
Number of Reactions: 1,600 Reactions

Contents & storage

• 16 x 1.25 mL ABsolute QPCR Mix (2X)

Store at -20°C


Manuals & protocols