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ABsolute QPCR Mix, SYBR Green, fluorescein

Catalog number: AB1220A

Thermo Scientific™  Related applications: Real Time PCR (qPCR)

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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of ABsolute QPCR Mix, SYBR Green, and added additional capabilities for your gene expression analysis.

Thermo Scientific ABsolute qPCR SYBR Green Mixes are optimized for SYBR Green chemistry and contain all the components necessary to perform quantitative PCR, with the exception of template and primers. The 2X qPCR SYBR Green Mix contains optimal levels of active SYBR Green I dye and Thermo-Start DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets.

ABsolute qPCR SYBR Green Mixes are available with the option of included high ROX reference dye concentration, Low ROX concentration, Fluorescein or no reference dye. A master mix optimized for capillary instruments is also available.


• High Sensitivity & Specificity—The master mixes contain our patented hot start enzyme Thermo-Start DNA Polymerase
• Optimised buffer containing SYBR Green I
• Reproducibility—All master mixes undergo extensive QC to ensure batch to batch consistency
• Broad dynamic range


• Gene expression analysis
• siRNA validation
• Genotyping

Related Products
ABsolute QPCR Mix, no ROX
ABsolute QPCR Mix, low ROX
ABsolute QPCR Mix, ROX
ABsolute QPCR Capillary Mix
ABsolute QPCR Mix, SYBR Green, no ROX
ABsolute QPCR Mix, SYBR Green, low ROX
ABsolute QPCR Mix, SYBR Green, ROX
ABsolute QPCR Mix, SYBR Green, fluorescein
ABsolute QPCR Capillary Mix, SYBR Green
For Research Use Only. Not for use in diagnostic procedures.


Detection Method: SYBR
For Use With (Equipment): BioRad MyiQ, BioRad iCycler iQ, BioRad iQ5
Reaction Speed: Standard
PCR Method: qPCR
Sample Type (General): DNA, dsDNA
Number of Reactions: 400 Reactions
Polymerase: Taq DNA Polymerase
Product Line: ABsolute
Product Size: 400 reactions

Contents & storage

• 1 x 5 mL ABsolute QPCR SYBR Green Fluorescein Mix (2X)
•100 µL MgCl2 (1M)
Sufficient for 400 x 25 µL reactions.

Store at -20°C


Manuals & protocols

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