ABsolute QPCR Mix, ROX

Catalog number: AB1139A

Thermo Scientific™  Related applications: Real Time PCR (qPCR)

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Description

Thermo Scientific ABsolute qPCR Mixes are optimized for use with hybridization probes and contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes. The 2X master mix contains a proprietary reaction buffer that provides high sensitivity, specificity, and reproducibility. ABsolute qPCR Mixes are available with the option of included high ROX reference dye concentration, Low ROX concentration, and with no ROX reference dye. There is also a master mix version optimized for capillary instruments.

Highlights

• High sensitivity & specificity - The master mixes contain our patented hot start enzyme Thermo-Start DNA Polymerase
• Reproducibility - All mixes undergo extensive QC to ensure batch to batch consistency
• Optimised buffer - For maximal performance in multiplex reactions

Applications
• Gene expression analysis
• siRNA validation
• Genotyping

Related Products
ABsolute QPCR Mix, no ROX
ABsolute QPCR Mix, low ROX
ABsolute QPCR Mix, ROX
ABsolute QPCR Capillary Mix
ABsolute QPCR Mix, SYBR Green, no ROX
ABsolute QPCR Mix, SYBR Green, low ROX
ABsolute QPCR Mix, SYBR Green, ROX
ABsolute QPCR Mix, SYBR Green, fluorescein
ABsolute QPCR Capillary Mix, SYBR Green
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: Primer-Probe Detection
For Use With (Equipment): 7000 System, 7300 System, 7700 System, 7900HT System, StepOne™ System, StepOnePlus™ System
Reaction Speed: Standard
PCR Method: qPCR
Sample Type (General): DNA, dsDNA
Number of Reactions: 400 Reactions
Polymerase: Taq DNA Polymerase
Product Size: 400 reactions

Contents & storage

Contains:
• 1 x 5 mL ABsolute QPCR ROX Mix (2X)

Store at -20°C

Documents

Manuals & protocols