Thermo Scientific™

ABsolute QPCR Mix, ROX

Catalog number:  AB1139A

Related applications:

Real Time PCR (qPCR)

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Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of ABsolute QPCR Mix and added additional capabilities for your gene expression analysis.

Thermo Scientific ABsolute qPCR Mixes are optimized for use with hybridization probes and contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes. The 2X master mix contains a proprietary reaction buffer that provides high sensitivity, specificity, and reproducibility. ABsolute qPCR Mixes are available with the option of included high ROX reference dye concentration, Low ROX concentration, and with no ROX reference dye. There is also a master mix version optimized for capillary instruments.


• High sensitivity & specificity - The master mixes contain our patented hot start enzyme Thermo-Start DNA Polymerase
• Reproducibility - All mixes undergo extensive QC to ensure batch to batch consistency
• Optimised buffer - For maximal performance in multiplex reactions

• Gene expression analysis
• siRNA validation
• Genotyping

Related Products
ABsolute QPCR Mix, no ROX
ABsolute QPCR Mix, low ROX
ABsolute QPCR Mix, ROX
ABsolute QPCR Capillary Mix
ABsolute QPCR Mix, SYBR Green, no ROX
ABsolute QPCR Mix, SYBR Green, low ROX
ABsolute QPCR Mix, SYBR Green, ROX
ABsolute QPCR Mix, SYBR Green, fluorescein
ABsolute QPCR Capillary Mix, SYBR Green


For Use With (Equipment): 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Product Line: ABsolute
Sample Type: DNA, dsDNA
Concentration: 2 X
Detection Method: Primer-Probe Detection
Fidelity (vs. Taq): 2 X
For Use With (Application): Real Time PCR (qPCR)
GC-Rich PCR Performance: High
No. of Reactions: 400 Reactions
No. of Tests: 400 Reactions
PCR Method: qPCR
Polymerase: Taq DNA Polymerase
Product Type Specs: qPCR Mix
Reaction Speed: Standard
Sufficient For: 400 Reactions
Volume: 5 mL

Contents & storage

• 1 x 5 mL ABsolute QPCR ROX Mix (2X)

Store at -20°C