Thermo Scientific™

Verso 1-step RT-qPCR Kit, with separate ROX vial

Catalog number: AB4100C
Thermo Scientific™

Verso 1-step RT-qPCR Kit, with separate ROX vial

Catalog number: AB4100C
Catalog Number
AB4100C
also known as AB-4100/C
Unit Size
400 reactions
Price (USD)
Price: 894.00
Your Price:
Availability
***
Quantity
Catalog Number
AB4100A
also known as AB-4100/A
Unit Size
200 reactions
Price (USD)
Price: 493.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
AB4100C
also known as AB-4100/C
400 reactions
Price: 894.00
Your Price:
***
AB4100A
also known as AB-4100/A
200 reactions
Price: 493.00
Your Price:
***
Product Overview
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The Thermo Scientific Verso 1-step RT-qPCR Kit for probe chemistry offers a quick and simple way to detect mRNA. The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures. This version of the kit comes with a separate vial of ROX reference dye.

Features of the Verso 1-step RT-qPCR Kit:

• Eliminate the need for DNase treatments—RT Enhancer (optional) prevents gDNA carry-over
• RT and qPCR steps performed in single tube—convenience and reduced contamination risk
• Use of the entire RT reaction as template for qPCR—improved sensitivity
• Verso RT enzyme is highly sensitive with broad dynamic range—accurate detection of RNA from 1 pg-1 ug
• Verso RT enzyme has a wide working temperature range (42 to 57°C)—improved success with GC-rich and other difficult templates
• Contains an inert blue dye for ease of pipetting during reaction set up

An optional RT Enhancer is also provided and can be used during the RT step to remove any DNA contamination, eliminating the need for DNase I treatment. All Verso 1-step RT-qPCR kits incorporate an inert dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.

In a 1-step approach to RT-qPCR, both the RT step and qPCR steps are performed in a single tube (1-step RT-qPCR). Adopting a 1-step approach is ideal when no cDNA storage is necessary for subsequent experiments. The approach minimizes the risk of contamination and improves reproducibility by reducing sample handling.

Applications

• Gene expression analysis
• RNAi validation
• Microarray validation
• Pathogen detection
• Genetic testing
• Disease research

Related Products
Verso 1-step RT-qPCR Mix, low ROX
Verso 1-step RT-qPCR Mix, ROX
Verso 1-step RT-qPCR Kit, with separate ROX vial
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application)
1-Step RT-qPCR
Detection Method
Primer-Probe
Format
Tube
GC-Rich PCR Performance
High
No. of Reactions
400 Reactions
PCR Method
1-step RT-qPCR
Sample Type
RNA
For Use With (Equipment)
BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, MJ Chromo4, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, MJ Opticon
Product Line
Verso
Product Type
1-Step RT-qPCR Kit
Sufficient For
400 Reactions

Contents & Storage

Contains:
•1 x 100 μL Verso Enzyme Mix
•1 x 500 μL RT enhancer
•1 x 5 mL 2X 1-Step qPCR Mix
•1 x 25μL ROX, 1mM
Sufficient for 400 x 25 μL reactions.

Store at -20°C

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