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View additional product information for EnzChek™ Reverse Transcriptase Assay Kit - FAQs (E22064)
2 product FAQs found
To expand the upper end of the PicoGreen’s dynamic range, simply increase dye concentration. By doubling the concentration, the dynamic range will double, etc. Keep in mind, however that increasing the dye concentration sacrifices the lower end of the assay due to increasing background fluorescence.
The assay should work with any reverse transcriptase.
Note: This is a solution reaction, so the reverse transcriptase needs to be available in solution to work, not encapsulated.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.