Invitrogen™

Anza™ 12 XbaI

Catalog number: IVGN0126
Have Questions?
Invitrogen™

Anza™ 12 XbaI

Catalog number: IVGN0126
Have Questions?
Catalog Number
IVGN0126
Unit Size
2000 units
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
12,000 units
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
IVGN01262000 units
-
IVGN012812,000 units
-
Product Overview
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Invitrogen™ Anza™ 12 XbaI is a restriction enzyme that cuts DNA at this recognition site: T^CTAGA, completely digesting the DNA in 15 minutes at 37°C.

For superior convenience, a single buffer and protocol are used with all Anza™ restriction enzymes. The single Anza buffer allows multiple restriction enzymes to be used simultaneously in one reaction. This helps eliminate the need for sequential digests and the DNA clean-up necessary when changing buffers. Additionally, the Anza buffer and restriction enzymes have been designed for flexibility in digestion times. Digestions of up to 16 hours can be carried out without star activity.

Convenient—all Anza enzymes are 100% functional in the single Anza buffer
Fast—complete digestion in 15 minutes
Flexible—overnight digestion without star activity
Simple—a single digestion protocol for all DNA types
Complete system—incorporates Anza DNA Modifying Enzymes for a complete cloning experience

Anza DNA Modifying Enzymes include the Anza T4 DNA Ligase Master Mix, Anza Alkaline Phosphatase Kit, Anza T4 PNK Kit, Anza DNA Blunt End Kit, and Anza DNA End Repair Kit. Use these enzymes for a complete cloning workflow.

Anza Red Buffer, which contains a density gradient and tracking dyes, is supplied for added convenience. Digestion reactions are as efficient in the Anza Red Buffer as in the Anza Buffer. Use of the Anza Red Buffer helps reduce pipetting steps when preparing multiple digestion reactions and a subsequent step includes agarose gel electrophoresis. For applications that require product analysis by fluorescence excitation, the colorless Anza Buffer without the tracking dyes is recommended.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Shipping Condition
Approved for shipment on Wet or Dry Ice
Methylation Sensitivity
Dam Methylation-Sensitive, Not CpG Methylation-Sensitive, Not Dcm Methylation-Sensitive
Enzyme
Xba I
Compatible Buffer
Anza™ 10X Red Buffer, Anza™ 10X Buffer
Sensitive to Heat Inactivation
Yes
Optimal Reaction Temperature
37°C
Type IIS RE
No
Quantity
2000 U
Product Type
Restriction Enzyme Cloning System
Research Category
Traditional Cloning
Product Line
Anza™

Contents & Storage

200 μL Anza 12 XbaI
500 μL Anza Buffer (10X)
500 μL Anza Red Buffer (10X)

Store at -20°C.

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