Thermo Scientific™

Maxima SYBR Green/ROX qPCR Master Mix (2X)

Catalog number: K0221
Thermo Scientific™

Maxima SYBR Green/ROX qPCR Master Mix (2X)

Catalog number: K0221
Catalog Number
K0221
Unit Size
200 reactions
Price (USD)
206.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
1,000 reactions
Price (USD)
874.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
4,000 reactions
Price (USD)
2,450.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K0221200 reactions
206.00
Your Price:
***
K02221,000 reactions
874.00
Your Price:
***
K02234,000 reactions
2,450.00
Your Price:
***
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Recommendations
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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Maxima SYBR Green qPCR Master Mix and added additional capabilities for your gene expression analysis.

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Thermo Scientific Maxima SYBR Green qPCR Master Mix is a ready-to-use solution optimized for qPCR and 2-step RT-qPCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG) (1). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most most real-time thermal cyclers (see Resources for a compatibility chart).

Highlights

SpecificityMaxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
Sensitivity—detects low copy number targets
Wide linear range—accurate quantification across 9 orders of magnitude
Reproducibility and convenience—ready-to-use 2x master mix

Applications

• Gene expression
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Maxima SYBR Green/ROX qPCR Master Mix (2x) includes Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer supplemented with ROX passive reference dye. Supplied with nuclease-free water.

Related Products
Maxima SYBR Green/ROX qPCR Master Mix (2X)
Maxima SYBR Green/Fluorescein qPCR Master Mix (2X)
Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial

Specifications

Concentration
2X
Detection Method
SYBR
Format
Tube
GC-Rich PCR Performance
High
No. of Reactions
200 reactions
PCR Method
qPCR
Polymerase
Taq DNA Polymerase
Reaction Speed
Standard
Sample Type
DNA, dsDNA
Volume
2 x 1.25 mL
For Use With (Application)
Real Time PCR (qPCR)
For Use With (Equipment)
7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Product Line
Maxima
Product Type
Real Time PCR SYBR Master Mix
Sufficient For
200 Reactions of 25 μL

Contents & Storage

Contains:
• 2 x 1.25 mL Maxima SYBR Green/ROX qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase, dNTPs (also dUTP) and SYBR Green I in an optimized PCR buffer with ROX passive reference dye; sufficient for 200 x 25 µL reactions.
• 2 x 1.25 mL Nuclease-Free Water

Store at -20°C.

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